Vanillin is a widely used flavor compound in the food, cosmetic, and pharmaceutical industries. Although microbial biosynthesis offers a green alternative to conventional chemical synthesis, challenges such as unstable heterologous enzyme expression, insufficient methyl donor supply, and product cytotoxicity limit de n...
Ming-Yue Tian, Jin-Yi Li, Yong Du et al.· Synthetic and Systems Biotec...· 0 citations
Indole-3-acetonitrile (IAN) and its derivatives-indole-3-carboxaldehyde (I3A), indole-3-carboxylic acid (I3CA), and 3,3'-diindolylmethane (DIM)-are high-value indole-derived compounds widely used in the chemical, pharmaceutical, and nutraceutical industries. However, their de novo microbial production has not yet been...
Meng-Chu Sun, Xiao-Lin Shen, Xin-Xiao Sun et al.· Trends in Biotechnology· 0 citations
Vanillyl alcohol (VNA) is a high-value compound used in the pharmaceutical industry and other fields. Its microbial de novo biosynthesis is severely limited by the low catalytic efficiency of O-methyltransferase. Here, we constructed a V314I mutant of caffeate O-methyltransferase via structure-guided protein engineer...
Jin-Yi Li, Yong Du, Jia Wang et al.· ACS Synthetic Biology· 0 citations
This work establishes an efficient E. coli chassis for sustainable BIAs production by systematically tackling flux imbalance and enzyme solubility and combined solubility-enhancing strategies.
Xia-Xia Zhang, Muhammad Hassan Butt, Wen Zhang et al.· ACS Synthetic Biology· 0 citations
Methyl salicylate (MeSA) is a volatile methylated aromatic ester widely used in agriculture, food, and pharmaceuticals. Microbial biosynthesis offers a sustainable alternative to plant extraction and petrochemical synthesis; however, efficient MeSA production remains limited by the low catalytic efficiency of carboxyl...
Yuan Gao, Shi-Bo Guo, Jun Wu et al.· Bioresource Technology· 0 citations
Construction of microbial cell factories often requires extensive reconfiguration of metabolic networks, which frequently compromises cell growth. Here, we engineered E. coli to produce dencichine (β-ODAP), a plant-derived hemostatic agent. Knocking out serB blocks the competing L-serine pathway, causing L-serine auxot...
Yu Zhang, Jun Wu, Jinghua Yu et al.· The Innovation Life· 0 citations
Daphnetin is a clinically established coumarin, but its native biosynthetic pathway remains elusive. In this study, a 2-oxoglutarate-dependent dioxygenase (2OGD), AtS8H, was identified that hydroxylates umbelliferone (UMB) to daphnetin , enabling the design of an artificial biosynthetic pathway. However, the pathway ef...
Shunmin Ji, Chong Xie, Yanyan Wang et al.· ACS Synthetic Biology· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
