Ya-Jun Wang

Engineering an (R)-Selective Transaminase for High-Substrate-Loading Asymmetric Amination of a Sterically Demanding N-Heterocyclic Ketone.

The biocatalytic asymmetric amination of bulky N-heterocyclic ketones is often limited by low enzyme activity and poor performance at high substrate loading. Herein, we developed an efficient (R)-selective transaminase for the synthesis of (R)-1-Boc-3-aminopiperidine through an integrated strategy combining phylogeny-g...