CRISPRoff and CRISPRon Epigenetic Editing: Molecular Mechanisms and Therapeutic Applications
Epigenetic modification plays a key role in gene regulation. CRISPRoff and CRISPRon, based on Nuclease-dead Cas9 (dCas9), can achieve precise, reversible regulation of gene expression without changing the DNA sequence, which is better than traditional cutting technology. This article systematically compares the two set...