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Zhao-Rong Chen

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#protein folding Oct 2026

Directed Evolution of a Protein Catenane Reveals How Noncanonical Topology Modulates Sequence–Property Relationships

Single-domain protein catenanes, in which two mechanically interlocked polypeptide rings fold into a compact structure, provide a tractable system for probing how chemical topology reshapes protein fitness. Using single-domain catenated dihydrofolate reductase (cat-DHFR) as a model, we combined homologue expansion, d...

Jing Fang, Zhao-Rong Chen, Ya-Jie Liu et al. · 0 citations

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