Structural basis and rational engineering of a thermophilic Cas13a for robust RNA detection.
Cas13a enzymes are particularly attractive for nucleic acid detection due to their robust collateral activity. Recently, there has been a surge in the discovery and engineering of thermostable Cas13a proteins for nucleic acid detection. The thermostable Cas13a from Thermoclostridium caenicola (TccCas13a) was identified...