Zhi-Qiang Liu

Fine-Tuning l -Arginine Biosynthesis and Bidirectional Dynamic Control Overcome Metabolic Bottlenecks for High-Titer l -Ornithine Production in Escherichia coli

l-Ornithine is a versatile amino acid with broad applications in the pharmaceutical, nutraceutical, and food industries. However, its efficient microbial production is constrained by a critical trade-off: excessive blockage of the downstream l-arginine pathway severely impairs cell growth, while intracellular l-ornit...

Key enzyme optimization and multi-node metabolic flux regulation drive l-arginine production in Escherichia coli.

Microbial production of l-arginine is often constrained by tight metabolic regulation and insufficient precursor supply. Here a plasmid-free, non-auxotrophic Escherichia coli strain for high-level production of l-arginine was rationally engineered, based on our previous constructed strain G0 with 12.4 g/L l-arginine pr...

[Machine learning-based optimization strategies for fermentation processes].

This paper systematically reviewed the latest research progress on the closed-loop intelligent fermentation control systems, spanning from underlying data perception to high-level decision-making and the transformation of fermentation control from traditional "macroscopic feeding regulation" to "microscopic metabolic p...

Enhanced d -Pantothenic Acid Biosynthesis in Corynebacterium glutamicum by the CPSPC System and Spatial Organization Strategy

d-Pantothenic acid (D-PA) is an essential precursor of coenzyme A and the acyl carrier protein. Its biosynthesis requires abundant precursors and precise regulation of the multifunctional enzyme, ketopantoate reductase. Here, we engineered Corynebacterium glutamicum for enhanced D-PA production through metabolic engi...

Combinatorial Metabolic and Process Engineering for Enhanced GA4+7 Production and Tunable GA4/GA7 Ratio in Fusarium fujikuroi.

This study demonstrated that the GA4/GA7 ratio can be modulated through both molecular and fermentation strategies, and engineering a high-yielding GA4+7 producer using this Δp450-3 mutant as a platform.

Reconstruction and metabolic regulation of O-succinyl-L-homoserine biosynthesis pathway in Escherichia coli W3110.

The plasmid-free system constructed in this study effectively avoids the plasmid-induced metabolic burden and genetic instability and demonstrates the prominent advantages and great application potential of plasmid-free modular engineering for the efficient biosynthesis of OSH and other high-value amino acids.

Enhancing d-Pantothenic Acid Production in Corynebacterium glutamicum via Integrating Dynamic Regulation With Structure-Guided Protein Engineering of Ketopantoate Reductase.

This study establishes a robust chassis for sustainable DPA production and provides a generalizable framework for engineering other valuable biochemicals.

Enhanced Adenosine Biosynthesis in Paecilomyces hepiali Through Strain Evolution and Transcriptomic Insights.

Adenosine is a major bioactive nucleoside and quality marker in cordyceps-derived fungal products, but its efficient biosynthesis in Paecilomyces hepiali remains limited by insufficient strain performance and incomplete understanding of metabolic regulation. Here, we developed an integrated strategy combining protoplas...

Transaminases engineering and their cascade systems for natural product synthesis.

Beyond direct pathway engineering: reprogramming Fusarium fujikuroi from a GA3 producer into a GA4+7 factory.

This study achieves indirect metabolic reprogramming not via conventional pathway engineering, but by targeting a membrane transport bottleneck and employing a putative post-transcriptional silencing mechanism.

Active Pocket Engineering of d-Tagatose 4-Epimerase for Improved Catalytic Performance and Efficient Cascade Synthesis of d-Tagatose from d-Glucose.

d-Tagatose is a rare hexose sugar with excellent properties, and its synthesis catalyzed by d-tagatose 4-epimerase (T4E) represents a competitive novel pathway. In this study, EbT4E derived from the Eubacteriales bacterium was screened and systematically characterized. By reshaping the microenvironment of the active po...