Author

Zhi-Wei Zhu

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#gene editing Sep 2026

Highly efficient production of 1,3-propanediol by CRISPR-Cas12a-edited Klebsiella pneumoniae using glycerol and lignocellulosic hydrolysate as co-substrates.

Efficient biosynthesis of 1,3-PDO, a key bio-based chemical, depends on precise regulation of the host metabolic network. In this study, a heterologous CRISPR-Cas12a genome editing system was established and systematically optimized in Klebsiella pneumoniae, enabling efficient and stable genome editing (75-100% efficie...

Li Wang, Ming-Yang Zhao, Yuan-Ming Ye et al. · Bioresource Technology · 0 citations

Aug 2026

Systems metabolic engineering of Escherichia coli for high-level branched-chain fatty acid production via dual precursor pathways.

Branched-chain fatty acids (BCFAs), naturally synthesized by Gram-positive bacteria, are promising feedstocks for the production of advanced biofuels. However, efficient BCFA biosynthesis in Gram-negative bacteria such as Escherichia coli remains challenging because of insufficient supply of branched-chain acyl-CoA pre...

Meng-Fan Hu, Cai Feng, Ming-Jun Li et al. · Metabolic Engineering · 0 citations