Zhuang Shao

Intramolecularly quenched fluorogens enable multispectral activation with a fluorescein-targeting single-chain variable fragment.

Fluorogen-activating proteins enable low-background fluorescence imaging by inducing emission only upon fluorogen binding. However, spectral diversification often involves coordinated optimization of fluorogens and their protein partners, while compatibility with structurally diverse fluorophore scaffolds remains limit...