gene editing

CRISPR and Gene Augmentation Rescue Trabecular Meshwork Dysfunction in iPSC Models of Lowe Syndrome

The results showed that AAV2-OCRL demonstrated the highest transduction efficiency in patient iPSC-derived trabecular meshwork models (iHTM) among the three AAV-OCRL vectors evaluated, establishing it as a promising delivery vector.

Molecular characterization of vitellogenin and its receptor with CRISPR-based sgRNA validation in the legume pod borer, Maruca vitrata (Geyer) (Lepidoptera: Crambidae).

This study provides the first CRISPR-oriented functional characterisation and sgRNA validation of the M. vitrata Vg gene, together with structural characterisation of VgR and Vg-VgR interaction analysis, providing preliminary molecular resources for future CRISPR/Cas9 studies and supporting future embryo microinjection...

Generation of Herbicide‐Resistant Alfalfa Germplasm via Prime Editing

ABSTRACT Alfalfa (Medicago sativa) is the most widely cultivated forage crop worldwide, owing to its high protein content, balanced amino acid profile, and abundance of vitamins and minerals. However, weed infestation severely threatens alfalfa yield and quality. The lack of herbicide‑resistant cultivars, combined with...

Mapping the Management-to-Cure Transition in Thalassemia Research: Semantic Topic Modeling of 17,651 Publications Across Three Decades.

The course of a major therapeutic transition is charted and carry direct implications for research funding allocation, global strategies for equitable access to curative therapies, and computational literature surveillance to inform clinical guideline updates.

Parthenocarpy in Cucurbitaceae: Genetic Regulation, Hormonal Crosstalk, and Breeding Implications

This review summarizes current knowledge of the genetic control, hormonal regulation, and breeding use of parthenocarpy in Cucurbitaceae and outlines the key questions that remain to be resolved for the development of stable, high-yielding parthenocarpic cultivars.

Making base editing accessible: Evaluating computational workflows for ABE-mediated gene knockout

EMERGING TRENDS IN STEM CELL THERAPY FOR BLOOD DISORDERS: FROM BLOOD BANKING TO HEMATOLOGICAL MALIGNANCIES

A thorough summary of emerging trends in SCT for blood disorders is offered, with emphasis on blood banking, transplantation strategies, therapeutic applications, and recent technological innovations shaping modern hematological practice.

Stem-like and effector peripheral helper T cells orchestrate distinct local immune responses in rheumatoid arthritis 2262732

It is indicated that interactions between S-Tph and B cells within TLSs of RA synovium are crucial for sustaining Tph population by promoting S-Tph self-renewal and differentiation into E-Tph cells.

Comparative transcriptomics and scarless genome editing uncover a periplasmic c-type cytochrome important for extracellular electron uptake in Thioclava electrotropha ElOx9T

The genetic basis for EEU in T. electrotropha is explored and a novel and potentially horizontally transferred mechanism for bacterial EEU is established, with implications for improved engineering of bioelectrochemical systems or use of novel electrotrophic microorganisms.

Fruit-Specific Promoters in Plants: Advances, Regulatory Mechanisms and Applications in Plant Biotechnology

This review systematically summarizes recent advances in FSP research and highlights the major challenges limiting the application of FSPs, including the relatively weak transcriptional activity of natural promoters, insufficient tissue specificity, and limited cross-species applicability.

Fish Germline Stem Cells: Biological Traits, Culture Techniques, Germplasm Repositories and Application Prospects

This review elaborates the biological characteristics and classification of fish GSCs, summarizes the regulatory mechanisms of GSC fate determination, and comprehensively updates the research progress of in vitro culture technology and germplasm repository development.

Oncogenic PIK3CA enhances collective migration of mammary epithelial cells through ERK wave propagation.

Analysis of factors regulating collective cell migration of genome-edited MCF10A cell lines carrying hotspot PIK3CA mutations suggest that polarized actomyosin contractility and pulsatile ERK activation must be constrained in the territory of a phase diagram compatible with mechanotransduction of ERK waves across cell-...