gene editing

Wheat Rust Diseases: Global Impact, Integrated Disease Management, and Emerging Biotechnological Innovations for Sustainable Crop Protection

Simple Summary Wheat feeds a large share of the world’s people, yet every growing season it faces a persistent enemy: a group of fungal diseases known as rusts. These fungi travel long distances on the wind, multiply quickly, and keep changing in ways that allow them to defeat the protection bred into wheat plants. Whe...

Programming primary human T cells to deliver gene editing machinery

Clinical applications of gene editors including CRISPR-Cas9 are limited by the cell-type specificity and tissue penetrance of current in vivo delivery options. Cell-mediated gene editor delivery could overcome these obstacles. To date, systems utilizing immortalized cell lines have demonstrated gene editor delivery in...

Current Evidence on Gene-Based and Epigenetic Therapeutic, Diagnostic, and Prognostic Strategies in Epithelial-Derived Carcinomas: A Scoping Review

Background: Epithelial-derived carcinomas exhibit genomic instability due to genetic mutation and epigenetic dysregulation. Mechanisms such as DNA methylation, histone modifications, and noncoding RNA regulation may lead to tumor progression, immune evasion, and treatment resistance. Presently, gene-based and epigeneti...

Complete assembly of Onobrychis viciifolia mitochondrial genome reveals evolutionary dynamics

Pushing the Boundaries of Knowledge in Alpha-1 Antitrypsin Deficiency.

Cutting-edge preclinical and translational developments with potential to redefine AATD management and move toward curative approaches are highlighted, highlighting cutting-edge preclinical and translational developments with potential to redefine AATD management.

Male reproductive genes influencing mating and fertility: enhancing the sterile insect technique and genetic control of Diptera pests.

A synthesis of male reproductive genes and regulatory pathways in Diptera according to the reproductive processes they control and the failure modes caused by their disruption can inform target prioritization, strain maintenance, and quality control of irradiated males, thereby enhancing the refinement of SIT and relat...

Repurposing Cas12a2-mediated abortive infection for efficient and low-toxicity microbial genome editing

CRISPR-Cas systems have revolutionized microbial genome engineering, yet counter-selection strategies typically rely on targeted DNA cleavage, where the severe cytotoxicity of double-strand breaks restricts transformation efficiency and clonal recovery. Here, we present an innovative strategy, SuCas12a2-Universal Recom...

Dynamic phosphorylation of DGCR8 regulates embryonic stem cell differentiation and microprocessor function

The Microprocessor complex, minimally composed of DROSHA and DGCR8, is ubiquitously required for microRNA biogenesis. It also regulates the balance between pluripotency and differentiation through mRNA alternative splicing and mRNA cleavage. However, how these various activities are regulated remains incompletely under...

PTPN22 as a novel therapeutic target: a key intracellular checkpoint for NK cell therapy

Abstract Background PTPN22, a protein tyrosine phosphatase (PTP) family member, has recently emerged as an intracellular regulator in adaptive immune cells. However, its function in natural killer (NK) cells remains unclear despite its high expression in innate immune cells. Methods We analyzed single-cell RNA-sequenci...

Identification of Novel Regulators of Telomere Length and its Impact on Endothelial Dysfunction

Telomere length (TL) is highly heritable and associated with a plethora of health conditions, including cancer, Alzheimer's and coronary artery disease (CAD). A number of studies have linked TL to CAD, with short TL preceding disease onset. Genetic studies support a causal link between the two, though the mechanism rem...

Post-transcriptional regulation of Influenza A virus mRNAs

The fate of cellular mRNAs is governed by intricate and dynamic post-transcriptional regulatory mechanisms that fine-tune cellular gene expression. Processes such as stabilisation, degradation, splicing, nuclear export and RNA editing, are essential for mRNA processing and are known to be hijacked by RNA viruses to enh...

Immuno-metabolic crosstalk in osteoarthritis: from mechanisms to therapies

The bidirectional crosstalk between immune and metabolic processes during OA progression is summarized and integrating multi-omics technologies with personalized medicine may improve patient stratification and dynamic monitoring and could facilitate the future development of more personalized and potentially disease-mo...