gene editing

Study of the genus Rosehip (Rosa) using molecular biological methods

This study presents a molecular genetic analysis of a rosehip sample using DNA barcoding and phylogenetic approaches. The aim of the research was species identification based on comparative analysis of nucleotide sequences of nuclear and chloroplast marker genes ITS, matK, rbcL, and trn. Genomic DNA was extracted from...

Targeted Mutations in d-ldh and budA Differentially Affect Adaptive Traits and Fermented Milk Quality in Companilactobacillus crustorum MN047

This study examined how targeted mutations in two pyruvate-associated genes, d-ldh and budA, affected adaptive phenotypes and fermented milk quality in Companilactobacillus crustorum MN047. Premature stop codons were introduced into d-ldh and budA by CRISPR-assisted base editing and verified by Sanger sequencing. The r...

Pathophysiology and Comprehensive Pharmacological Management of ATTR Cardiac Amyloidosis: Knowledge Gaps, Ongoing Clinical Trials, and Future Research Directions

Cardiac amyloidosis is an increasingly recognized cause of heart failure in the setting of progressive restrictive cardiomyopathy, particularly among older adults, males, and individuals of African descent, with remarkable advances in the therapeutic landscape, but substantial knowledge gaps persist.

PS10-4. Effect of Mutating Porcine CYP17A1 for Altering Pregnenolone Metabolism to Control Boar Taint.

Using a well-defined cell culture system to identify targets for producing gene edited pigs without boar taint could not only improve pork production but also give insight into a highly complex cytochrome P450 system.

Replication Data for: Rice leaf structural adjustment to sustain photosynthetic traits under drought and produce more grain: Evidence from field studies of the 3K indica panel

Understanding plant traits that contribute to maintenance of physiological activities under drought is crucial for sustainable rice production. Leaf morphological characters together with UAV-based HTP measurements of canopy temperature (CT) and NDVI, leaf water potential (ψLeaf), photosynthetic traits including A, gs...

Transcriptomic Reanalysis Reveals Complement, Circadian, and Toll-Like Receptor Signaling Remodeling in Lactiplantibacillus plantarum-Induced Innate Immune Memory in Human Monocytes

Findings point to a multi-layered adaptive response where L. plantarum not only dampens canonical inflammatory signaling but also actively remodels cellular processes related to pathogen recognition, metabolic sensing, and stress responses.

101. Assessment of Published Bovine Guide RNA Design Practices.

CRISPR/Cas9 is the most widely applied gene-editing (GnEd) technology in livestock, with more than 200 published studies reporting the generation of live, gene-edited animals for agricultural applications. This system consists of a Cas9 nuclease that cleaves DNA and a 19-20 base pair guide RNA (gRNA) that directs the...

2.72P Optimization of intramuscular administration and in-vivo monitoring of gene-editing therapy in Duchenne muscular dystrophy

bactiviz: A Lightweight Command-Line Tool for Linear Collinearity Visualization of Bacterial Genomes

Comparative visualization of bacterial genome structure is an important component of microbial genomics, enabling the inspection of genome conservation, rearrangements, inversions, insertions and deletions, and large-scale differences in gene organization. I present bactiviz, a lightweight command-line tool for generat...

Super-Optimized Transgenic Synthetic Gyno-Genome (SOSG-2026): A Cross-Species Synthetic DNA Blueprint and Transgenic Sperm-Mediated Gene Transfer (SMGT) Methodology

वैज्ञानिक आविष्कार: सुपर-ऑप्टिमाइज्ड ट्रांसजेनिक सिंथेटिक गाइनो-जीनोम (Super-Optimized Transgenic Synthetic Gyno-Genome) मुख्य आविष्कारकर्ता (Principal Inventor): बंटी कुमावत (Banti Kumawat) विषय: सिंथेटिक बायोलॉजी, जेनेटिक इंजीनियरिंग और क्रिसपर-मल्टीप्लेक्सिंग (CRISPR Multiplexing) उद्देश्य: अत्यधिक दूध उत्पादन, जलवा...

Super-Optimized Transgenic Synthetic Gyno-Genome (SOSG-2026): A Cross-Species Synthetic DNA Blueprint and Transgenic Sperm-Mediated Gene Transfer (SMGT) Methodology

वैज्ञानिक आविष्कार: सुपर-ऑप्टिमाइज्ड ट्रांसजेनिक सिंथेटिक गाइनो-जीनोम (Super-Optimized Transgenic Synthetic Gyno-Genome) मुख्य आविष्कारकर्ता (Principal Inventor): बंटी कुमावत (Banti Kumawat) विषय: सिंथेटिक बायोलॉजी, जेनेटिक इंजीनियरिंग और क्रिसपर-मल्टीप्लेक्सिंग (CRISPR Multiplexing) उद्देश्य: अत्यधिक दूध उत्पादन, जलवा...

Protocol for quantification of AAV-CRISPR-mediated hepatic gene editing in mice.

This protocol enables quantification of INDELs and DNA-SVs, allowing for validation of gene disruption, and describes guide RNA (gRNA) design, plasmid cloning, delivery of AAV-CRISPR to the liver, and execution of liver DNA-editing analysis.