Sep 2026· International Journal of Science and Research Archive· 0 citations
CRISPR and Genetic Engineering
TL;DR
This review discusses about the mechanism followed by CRISPR CAS9 in gene editing and various other possible applications of CRISPR CAS9.
Abstract
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR CAS9) system is a powerful tool in genome editing and has evolved as a core part of molecular biology, biotechnology, biomedical research, diagnostics and therapeutics and spreading its vastness in other fields like agriculture, life sciences, industrial biotechnology and synthetic biology. CRISPR CAS9 does not only repair the cellular DNA but also works as the internal defense mechanism by adapting, expressing, recognizing the target and performing actions. This review discusses about the mechanism followed by CRISPR CAS9 in gene editing and various other possible applications of CRISPR CAS9.
Clustered regularly interspaced short palindromic repeats (CRISPR) and associated (Cas) systems have revolutionized the field of genome engineering by providing versatile, efficient, and programmable tools for precise genetic manipulation. Originally identified as an adaptive immune mechanism in prokaryotes, CRISPR/Cas...
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This protocol covers both single-gRNA (sgRNA) and dual-gRNA directed knockout systems and describes the procedure for constructing an Auxin-Inducible Degron (AID) knock-in-mediated system to induce the rapid degradation of a target protein in cells, thereby investigating its function.
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Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and their associated (Cas) proteins have progressed from an obscure, unannotated repeat sequence first noted in Escherichia coli in 1987 to one of the most versatile toolkits in modern genetics and biochemistry. This chapter provides an expanded, detail...
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How CRISPR interference, CRISPR activation, base editing, base editing, and prime editing have improved the understanding of disease mechanisms, while creating new opportunities for therapeutic intervention are discussed.
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