In Vivo Anti‐Nociceptive Activity of Isoquercetin in Rats Supported by ADMET and Molecular Docking Analysis

Anti-nociceptive property of thymol: animal study with molecular docking and MD simulation studies

Phytochemical Characterization Using HPLC-DAD, Antiglycation Effect at Multiple Stages, Anti-Inflammatory and Analgesic Activities of Solanum elaeagnifolium Cav: Experimental and Computational Studies

Solanum elaeagnifolium has been utilized for its analgesic, anti-inflammatory, and antioxidant properties to treat a range of conditions, including pain and inflammation. It possesses antibacterial, insecticidal, and molluscicidal properties as well. To investigate the pharmacological potential of the S. elaeagnifolium extract, in vitro, in vivo, and in silico studies were used. Albumin denaturation, heat-induced anti-haemolytic action, and lipooxygenase inhibition were the three techniques used to test anti-inflammatory effectiveness. The phenolic chemicals utilized were identified through the use of high-performance liquid chromatography (HPLC). The effectiveness of the extract in lowering problems connected to diabetes was further evaluated by evaluating fructosamines, carbonyl groups, and β-amyloid formations in albumin glycation. Regarding in vivo studies, the Writhing test and the tail flick test were the two techniques used to evaluate analgesic activity. For docking analysis, we used the following identifiers: cyclooxygenase (PDB ID: 6COX), lipooxygenase (PDB ID: 3V99) and VC1 domain of the receptor for advanced glycation products (PDB ID: 7LMW). The most prevalent phenolic chemicals, according to HPLC analysis, were 3,4-dihydroxybenzoic acid (4.78%), caffeic acid (1.28%), gallic acid (8.18%), ursolic acid (2.6%), syringic acid (1.78%), quercetin (24.09%), catechin (6.67%), and p-coumaric acid (20.49%). Significant suppression of albumin glycation and fructosamine production was demonstrated by the extract, indicating that it may help lessen difficulties associated with diabetes. Furthermore, the extract demonstrated a strong anti-inflammatory impact, with an IC50 of 146 ± 1.17 μg/mL for lipooxygenase inhibition, 87.64 ± 5.35 μg/mL for anti-haemolytic action, and 86.94 ± 1.63 μg/mL for albumin denaturation inhibition. A similar analgesic effect to those of analgesic medications was also demonstrated by SEFE extract. Reinforcing the relevance of the observed effects, the in silico study of the tested activities validated these findings. These findings indicate significant pharmacological promise in the management of diabetes consequences, including inflammation and protein glycation, as well as an analgesic.

Anti-inflammatory and antinociceptive effects of a novel 1,5-benzodiazepine derivative in murine models.

MAL exhibits potent anti-inflammatory and analgesic properties, particularly at 25 mg/kg, reducing neutrophil-mediated inflammation, and represents a promising candidate for further pharmacological development.

Antinociceptive and Anti-inflammatory Efficacy of Oleic and Linoleic Acid Application

The present study aimed to comparatively evaluate the antinociceptive and anti-inflammatory effects of oleic acid (OA) and linoleic acid (LA), administered individually and in combination, and to investigate their impact on serum pro-inflammatory cytokines in an experimental animal model. A total of 96 rats were orally treated with OA (45–90 mg/kg), LA (45–90 mg/kg), OA + LA (45+45 mg/kg and 90+90 mg/kg), standard analgesics (indomethacin and acetylsalicylic acid, ASA), or vehicle control. The antinociceptive properties were examined through Tail Flick, Hot Plate, and writhing test induced by acetic acid injection, whereas the anti-inflammatory properties were examined through carrageenan-induced paw edema. The concentrations of TNF-α and IL-1β in serum were determined by ELISA. OA demonstrated statistically significant antinociceptive effects in both central and peripheral pain models, exhibiting efficacy comparable to ASA. In contrast, LA alone did not produce significant analgesic effects. Notably, co-administration of OA and LA attenuated the analgesic efficacy observed with OA alone. In the carrageenan-induced inflammation model, both OA and OA + LA significantly reduced paw edema, with effects comparable to indomethacin, whereas LA alone showed limited anti-inflammatory activity. Biochemically, both OA and LA significantly elevated serum TNF-α levels, while no significant differences were observed among groups in IL-1β levels. These findings suggest that OA possesses pronounced antinociceptive and anti-inflammatory properties. However, the reduced efficacy observed with combined administration and the elevation of cytokines indicate that OA and LA might work via different pathways, thus warranting further investigation into their dose-dependent interactions.

Anti-inflammatory and antinociceptive activity of indenopyrroles and indenopyridazines in the experiment

The studied compounds of the indenopyrrole series and their indenopyridazine derivatives are virtually non-toxic compounds, exhibit an anti-inflammatory effect and reduce the severity of pain associated with inflammation.

Evaluation of anti-arthritic activity of aripiprazole through multi models: new potential of an old drug.

It was evaluated in this study that aripiprazole showed inhibition of protein degradation and stability of human red blood cell membrane in concentration gradient manner, demonstrating maximum activity at concentration of 6400 µg/ml.