2026· Methods in molecular biology· Vol 3069, pp.
53-72
· 0 citations
Medicine
TL;DR
The UbiSite approach, a refined strategy for site-specific mapping of global ubiquitination using mass spectrometry-based proteomics, offers a robust and versatile platform for comprehensive ubiquitinome profiling with enhanced specificity and depth, enabling new insights into the regulatory roles of ubiquitin across diverse biological contexts.
This study demonstrates that integrating ubiquitomics provides a superior sensitivity layer for PROTAC safety assessment, capable of uncovering mechanism-based liabilities that escape conventional global proteomic screening.
Hongtao Wen, Hui Liu, Jinfang Liu et al.· Analytical Chemistry· 0 citations
SUMOylation is an essential eukaryotic ubiquitin-like post-translational modification that plays a central role in the regulation of various nuclear processes and stress responses. It canonically occurs at lysine residues within ΨKXE consensus motifs that lie in intrinsically disordered regions or loops and interact sp...
El Hadji Cisse, L. Visticot, Rafael Cepa et al.· bioRxiv· 0 citations
Experimental strategies to identify UID degrons that mediate degradation by the catalytic 20S proteasome particle are described and an integrated workflow-spanning degron mapping to functional validation-provides a generalizable framework for uncovering ubiquitin-independent degrons.
Y. Shaul· Methods in molecular biology· 0 citations
The discoveries suggest that during plant immunity, HECT-type ligases and the proteasomes they associate with, control cellular proteostasis by governing the stabilities of both E3 ligases and their substrates by controlling the stability of autoubiquitinating E3 ligases.
Zhi-Shuo Wang, Robert O. Mason, Heather Grey et al.· bioRxiv· 0 citations
This review compares midnolin-directed degradation with proteolysis-targeting chimeras and molecular glue degraders and identifies the design, delivery, and safety principles that will determine whether it becomes a useful ubiquitin-independent complement to existing degraders.
Yu-Wei Tang, Yin-Hao Zhou, Xin Zhang et al.· TIPS - Trends in Pharmacolog...· 0 citations
While protein ubiquitination has been extensively studied, the roles of unanchored ubiquitin and its chains remain less understood, largely due to a lack of specific, high-affinity tools for their enrichment and visualization. To address this, we used high-throughput affinity maturation and computational protein design...
Shi-Hua Shi, A. Bendel, G. Kempf et al.· Science Advances· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
