TL;DR
This work integrated the MutaT7 system, which uses two base deaminases, into phage-assisted evolution to direct all four transition mutations specifically to the GOI, promoting the accumulation of all transition mutations, providing greater diversification of the GOI.
This review will focus on three core strategies-traditional random evolution, global accelerated evolution, and targeted continuous evolution-systematically elucidating their developmental trajectories, applications in the construction of microbial cell factories, and future directions.
Targeted mutagenesis is a crucial tool for in vivo continuous evolution. However, existing methods for long-range targeted mutagenesis in vivo often face limitations such as low efficiency, poor host compatibility, operational complexity, and uneven distribution of mutations across these windows. In this study, we deve...
The results demonstrate that PEACE is an efficient, versatile, and robust platform for in vivo continuous evolution with broad potential in protein engineering.
It is proposed that MGE-driven fusion-deletion cycles represent a general principle of plasmid evolution, explaining the rapid emergence and persistence of multidrug-resistant plasmids across bacterial pathogens.
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