TL;DR
A comprehensive protocol for applying phage-assisted continuous evolution (PACE) strategy to select CP inhibitors against aggregation-prone proteins of interest is provided.
Cellular selection has emerged as a powerful approach for high throughput de novo discovery of bioactive cyclic peptides (CPs). This strategy coexpresses genetically encoded CP libraries with the target of interest in cells and uses customizable selection systems to link desired CP activities to a selectable phenotype...
Peptide phage display is a cornerstone technology in molecular engineering, providing a robust platform for the rapid discovery of high-affinity ligands. The rapid rise of peptides, especially in therapeutics, over recent years has shed light on the importance of phage display as a tool for peptide discovery. The evolu...
Cyclic peptides are powerful scaffolds for modulating protein–protein interactions; however, methods for constructing cyclic peptide libraries for cell-surface display applications remain limited. Here, we present a bacterial surface display platform that enables site-specific incorporation of diverse unnatural amino...
Macrocyclic peptides possess numerous advantages, including enhanced tissue penetration, low immunogenicity, good resistance to protease degradation, and high targeting specificity, making them increasingly attractive for drug development. Genetically encoded cyclic peptide libraries enable rapid and convenient de novo...
The first peptide affinity ligands targeting the baculoviral envelope glycoprotein GP64 for the purification of active Baculovirus particles are introduced, establishing SB4-POROS as a promising technology with a favorable projected cost structure for Baculovirus purification.
Anticancer peptides (ACPs) are frequently discussed as a single therapeutic class, yet the term encompasses molecules that perform markedly different jobs: direct tumor-cell killing, intracellular target modulation, tumor homing and penetration, or selective delivery of a separate payload. This functional diversity cre...
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