AI Networking Cookbook: Practical recipes for AI-assisted network automation and development
TL;DR
An optimized protocol for isolating vasculature-enriched protoplasts from mature Arabidopsis thaliana leaves is presented and the basic pipeline for downstream analysis is introduced, providing a reliable and reproducible method for isolate high-purity phloem-enriched protoplasts.
Single-cell technologies enable epigenomics characterization at single-cell resolution, offering novel insights into gene expression regulation and a wide range of biological processes, including cell fate determination, developmental differentiation, and environmental adaptation. In recent years, plant single-cell epi...
This protocol enables effective cell lysis, nucleic acid purification, and efficient removal of genomic DNA while preserving all transcript classes, including small regulatory RNAs (sRNAs), and provides a basis for future studies investigating the gene expression dynamics and regulatory networks of F. nucleatum under d...
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