The genome-wide profiling of histone modifications by CUT&Tag in cancer tissues, a relatively new method for profiling diverse chromatin components, is described.
Yang Liu, Paul A. Wade· Methods in molecular biology· 0 citations
A detailed CUT&RUN protocol from sample collection through data analysis, including best practices and defined controls to ensure specific, efficient, and robust target profiling is provided.
Tessa M. Firestone, Bryan J. Venters, Katherine Novitzky et al.· Methods in molecular biology· 0 citations
This chapter describes the experimental setup and provides a detailed protocol for performing chromatin immunoprecipitation in Ustilago maydis or related smut fungi.
L. Dittiger, Juliane Kühne, Christian Müller et al.· Methods in molecular biology· 0 citations
An optimized low-input Cleavage Under Targets and Release Using Nuclease (CUT&RUN) workflow tailored for fluorescence-activated cell sorting (FACS) isolated NCCs from zebrafish embryos is presented and can also offer insights into epigenetic dysregulation associated with disease.
A. Nasreen, Sribas Chowdhury, Vivek T. Natarajan· Methods in Enzymology· 0 citations
This chapter outlines the principles and experimental workflow of ChIPmentation, including crosslinking, chromatin fragmentation, immunoprecipitation, tagmentation, library preparation, sequencing, and data analysis, and discusses computational strategies for processing and analyzing ChIPmentation data.
Amalanandan Johna, Sreedarsanam Sreya, Sengupta Tiyas et al.· Methods in Enzymology· 0 citations
The protocols for ChIP-seq are outlined, including immunoprecipitation, library preparation for high-throughput sequencing, and bioinformatics analysis of sequencing data that the authors routinely perform in their lab groups, and include troubleshooting tips for each step.
Zachary Bigelow, S. Nechaev, Archana Dhasarathy· Methods in molecular biology· 0 citations
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