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2026

CUT&Tag for Chromatin Profiling in Cancer Tissue.

The genome-wide profiling of histone modifications by CUT&Tag in cancer tissues, a relatively new method for profiling diverse chromatin components, is described.

Yang Liu, Paul A. Wade · 0 citations
Open access 2026

High-Efficiency Genomic Mapping of Chromatin-Associated Targets with CUT&RUN.

A detailed CUT&RUN protocol from sample collection through data analysis, including best practices and defined controls to ensure specific, efficient, and robust target profiling is provided.

Tessa M. Firestone, Bryan J. Venters, Katherine Novitzky et al. · 0 citations
2026

Defining active and repressive chromatin states in neural crest cells using low-input CUT&RUN.

An optimized low-input Cleavage Under Targets and Release Using Nuclease (CUT&RUN) workflow tailored for fluorescence-activated cell sorting (FACS) isolated NCCs from zebrafish embryos is presented and can also offer insights into epigenetic dysregulation associated with disease.

A. Nasreen, Sribas Chowdhury, Vivek T. Natarajan · 0 citations
2026

ChIPmentation-based mapping of histone modifications in aging: Experimental design and analytical considerations.

This chapter outlines the principles and experimental workflow of ChIPmentation, including crosslinking, chromatin fragmentation, immunoprecipitation, tagmentation, library preparation, sequencing, and data analysis, and discusses computational strategies for processing and analyzing ChIPmentation data.

Amalanandan Johna, Sreedarsanam Sreya, Sengupta Tiyas et al. · 0 citations
2026

Chromatin Immunoprecipitation Sequencing in Cancer Cells.

The protocols for ChIP-seq are outlined, including immunoprecipitation, library preparation for high-throughput sequencing, and bioinformatics analysis of sequencing data that the authors routinely perform in their lab groups, and include troubleshooting tips for each step.

Zachary Bigelow, S. Nechaev, Archana Dhasarathy · 0 citations

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