TL;DR
An optimized low-input Cleavage Under Targets and Release Using Nuclease (CUT&RUN) workflow tailored for fluorescence-activated cell sorting (FACS) isolated NCCs from zebrafish embryos is presented and can also offer insights into epigenetic dysregulation associated with disease.
In mice, zygotic gene activation (ZGA) occurs in two waves: a minor wave at the 1-cell stage and a major wave at the 2-cell stage. At the 1-cell stage, chromatin is highly decondensed and transcriptionally permissive; however, global transcription is low. At the 2-cell stage, global transcription increases with chromat...
These findings reveal a previously unrecognized role for PRCs in establishing TAD-scale repressive chromatin domains during neuronal maturation, thereby safeguarding neuronal identity from external stimuli through broad silencing of alternative cell fate programs.
Ch chromatin polyvalency model is established as a multi-layered, hierarchical repression mechanism that governs temporal control of gene expression during embryogenesis and disrupts temporal control of polyvalent gene activation.
It is shown that loss of default neural differentiation capacity and failure to form brain organoids are linked to erosion of bivalent chromatin marks at developmental gene loci, independent of DNA methylation, driving acquisition of a posterior epiblast-like state and premature developmental gene expression.
This approach uncovers epigenomic priming of basal cells in the mammary gland and captures the dynamics of basal-to-luminal transdifferentiation, suggesting that epigenomic and transcriptional remodeling do not occur in complete synchrony during cell-fate conversion.
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