Characterization and genomic analysis of Bacillus halotolerans G3-2: a potential biocontrol agent against apple Alternaria leaf blotch disease.

Integrating biocontrol efficacy and genomic potential of Bacillus velezensis BJ-1141: from broad-spectrum antagonism to tomato Fusarium wilt suppression.

AIMS To isolate and characterize a rhizosphere-derived Bacillus velezensis strain with biocontrol and plant growth-promoting potential, and to evaluate its efficacy against plant pathogens together with its genomic basis. METHODS AND RESULTS A bacterial strain (BJ-1141) was isolated from the rhizosphere and identified as B. velezensis based on morphological characteristics and phylogenetic analyses of 16S rRNA and gyrA gene sequences. The strain exhibited broad-spectrum antagonistic activity against 24 plant pathogenic fungi, inhibiting 23 pathogens by more than 50%, with a maximum inhibition rate of 95.57%. BJ-1141 produced hydrolytic enzymes (amylase, protease, cellulase and pectinase) and plant growth-promoting factors, including siderophores and indole-3-acetic acid (IAA). It also demonstrated phosphorus solubilization, nitrogen fixation and biofilm formation. Greenhouse experiments showed that root application of BJ-1141 effectively controlled tomato Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici, achieving control efficacies of 81.13 - 85.93%, which were higher than those of chemical and commercial microbial treatments. In addition, BJ-1141 significantly increased plant height, biomass and chlorophyll content in tomato and lettuce seedlings. Whole-genome sequencing revealed a 3 902 965 bp genome containing 13 biosynthetic gene clusters associated with antimicrobial secondary metabolites, including surfactin, fengycin, bacillaene, difficidin, macrolactin H, bacillibactin and bacilysin. CONCLUSIONS B. velezensis BJ-1141 is a multifunctional bacterial strain with strong biocontrol efficacy and plant growth-promoting traits, supported by genomic evidence, and represents a promising candidate for sustainable crop disease management.

Genome Sequence Resource of Bacillus velezensis Strain H166, a Potential Biological Control Agent for Apple Replant Disease

The genome sequence provides important foundational data for further elucidating the molecular mechanisms underlying the biocontrol activity of B. velezensis strain H166 against Apple Replant Disease.

Systematic Investigation of the Biocontrol Potential of Bacillus velezensis B05 against Soybean Root Rot Pathogen Fusarium spp.

Results indicate that B. velezensis B05 has potential as a multifunctional biocontrol agent for sustainable management of soybean root rot.

Genomic insights into biosurfactant-mediated antifungal activity of native Bacillus amyloliquefaciens EG6.1 against Fusarium oxysporum

Overall, these results confirm that B. amyloliquefaciens EG6.1, in its new formulation consisting of pellet fractions and sonicated culture, shows great potential as a green biocide and contributes to the development of disease management strategies, while supporting SDG’s 2 and 12.

Whole-genome sequencing and characterization of Pseudomonas stutzeri P1 endophyte isolated from potato unveils plant growth-promoting and other traits

The potential of P1 as a promising bioinoculant candidate for sustainable agriculture in the potato sector is demonstrated and the genome lacked major virulence factors and antimicrobial traits, supporting the non-pathogenic nature of the P1 strain.

Genome-informed characterization and targeted mycotoxin screening of an Aspergillus niger starter candidate for hawk tea solid-state fermentation