A complete, gap-free genome of Medicago lupulina, a tough, high-protein forage legume, and a gene-editing tool for this species, which was used to alter its leaf shape, provide key resources for improving alfalfa and breeding better forage crops for high-altitude environments.
A CRISPR/Cas9 toolkit was established for the rice leaffolder using entirely endogenous regulatory elements and provides a system for functional genomics in this pest.
Jun-Chen Yan, Bing-Peng Liu, Hui Lu et al.· Insect Science· 0 citations
This targeted modification markedly improved rice eating and cooking quality while maintaining the levels of total starch, total protein, and key agronomic traits.
Zhihui Chen, Yu Chen, Gexing Wan et al.· Journal of Integrative Plant...· 0 citations
Agrobacterium
‐mediated transformation followed by regeneration without antibiotics‐dependent selection offers a a straightforward and effective approach for generating transgene‐free edited plants in grapevine, and the use of CRISPR/LbCas12a enables the simultaneous editing of multiple genes, thereby facilitating...
C. Ren, Yan-Ping Lin, Chen-Cong Liu et al.· Plant, Cell and Environment· 0 citations
Systematic comparison of Cas12i gene-editing tools in tomato identifies Cas-SF01 as the most efficient variant, achieving editing efficiencies comparable to conventional Cas9 at some genomic sites and providing a compact, highly specific, and versatile tool for studying gene function, engineering fruit metabolism, and...
Si-Qun Wu, Lei Zhu, Mei-Li Zhou et al.· Journal of Integrative Plant...· 0 citations
ABSTRACT Clonostachys rosea is a fungus with significant applications in biological control, plant growth promotion, and secondary metabolite production essential for agriculture. We report the de novo genome assembly of C. rosea strain CMAA1284 (also known as LQC 62), which shows high contiguity and excellent gene com...
P. Giachetto, A. Nhani, Wagner Bettiol et al.· Microbiology Resource Announ...· 0 citations
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