Unraveling Resistance and Thresholds in Phage Therapy Using In Vitro Staphylococcus aureus experiments and Pharmacokinetic/Pharmacodynamic Models.

Experimental evolution of phage K enhances antibacterial activity against USA300 MRSA in lung infection models

Findings highlight the value of experimental evolution for tailoring therapeutic phages and support phage adaptation as a promising strategy for developing interventions against multidrug-resistant S. aureus.

Modeling Phage–Antibiotic Synergy, Innate Immunity, and Phage Resistance in Multidrug-Resistant Acinetobacter baumannii

Background/Objectives: Antimicrobial resistance has been recognized as a major global health threat, with multidrug-resistant Acinetobacter baumannii identified as one of the most critical pathogens. To address the limitations of conventional antibiotics, phage therapy has been proposed as a complementary or alternativ...

Phage-first treatment results in subpopulation-driven collateral sensitivity to beta-lactams in an extensively drug-resistant Pseudomonas aeruginosa

How specific beta-lactams can be prioritized and optimized in phage-antibiotic combinations to maximize the antibacterial activity is shown, providing mechanistic insights into phage-induced collateral sensitivity of beta-lactams.

Harnessing phage-ciprofloxacin synergy to overcome multidrug-resistant infections and biofilm persistence

Phages targeting highly prevalent Greek multidrug-resistant Pseudomonas aeruginosa clinical isolates: In vitro and in vivo data.

Metallo-β-lactamase-producing Pseudomonas aeruginosa (MBL-PA) poses a major therapeutic challenge. In search of alternative therapeutic approaches, bacteriophages against MBL-PA were isolated from environmental and clinical samples and thoroughly characterized. Samples from a sewage treatment plant (n = 50), rectal swa...

Evaluation of Sequential Phage-Antibiotic Therapy Reveals Enhanced Biofilm Control with Meropenem and Colistin in Clinical MDR Hypervirulent Klebsiella pneumoniae Strain.

Sequential phage-antibiotic treatment was effective against MDR-HvKp biofilms, with pre-phage exposure enhancing antibiotic access through biofilm disruption, and the bacteriostatic nature of tigecycline reduced efficacy by affecting phage replication.