Aug 2026· Journal of Virological Methods· pp.
115450
· 0 citations· 20 references
Medicine
TL;DR
An indirect competitive ELISA (ic-ELISA) using a monoclonal antibody against recombinant σC protein is developed, suitable for large-scale ARV surveillance and vaccine monitoring.
Abstract
Avian reovirus (ARV) causes viral arthritis in poultry, and emerging variants in China demand improved serosurveillance. We developed an indirect competitive ELISA (ic-ELISA) using a monoclonal antibody against recombinant σC protein. After optimization (coating antigen 6.0μg/mL, mAb 1:4000, blocking 90min, serum 60min, mAb 90min), the cut-off percent inhibition was set at 44.5% based on 40 negative sera. The assay showed no cross-reactivity with six common avian pathogens, a detection limit of 1:400 serum dilution, and excellent reproducibility (intra-CV ≤0.70%, inter-CV ≤7.70%). Testing 87 field sera from various regions of China gave an overall seropositivity of 59.8% (regional range 50.0-75.0%). Compared with a commercial IDEXX kit, our ic-ELISA detected 52 positives vs. 48 (χ²=4.32, P=0.038), demonstrating superior sensitivity. This reliable, sensitive, and specific serological tool is suitable for large-scale ARV surveillance and vaccine monitoring.
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