A practical framework for selecting appropriate gene delivery strategies for microalgal synthetic biology is presented, emphasizing the need for delivery platforms compatible with diverse cargos, recipient species, and engineering applications.
Where genetic accessibility can be established, the integration of CRISPR technology with synthetic biology may enable more precise gene regulation and could support the development of next-generation engineered Bifidobacterium-based platforms.
This chapter emphasizes that the strategic selection of a delivery vehicle must be harmonized with the specific cargo and the unique biological requirements of the target tissue to ensure therapeutic success.
Bilge Debelec Butuner· Methods in molecular biology· 0 citations
ABSTRACT Polyplexes are versatile, efficient nonviral vectors for gene-based therapies and offer safer alternatives to conventional viral delivery systems. Delivering nucleic acids, including DNA, RNA, and siRNA, remains challenging because of limited stability, cellular uptake, and intracellular trafficking. Formed th...
Hamaira Liaqat, Huma Hameed, Syed Muhammad Ahmad et al.· Polymer-Plastics Technology...· 0 citations
This article reviews the recent progress and challenges associated with nano-based non-viral vectors designed for targeted delivery of DNA into cancerous cells.
M. Yousefian, Maryam Baharmast· Journal of Biomaterials Scie...· 0 citations
