It is found that translation inhibition by bacteriostatic antibiotics induces intracellular turgor pressure buildup by perturbing physiological balance in E. coli, showing bacteriostasis as a physiologically unstable state and providing a systems-level framework for its lethality, enabling the rational design of optimized therapies.
Bacterial proliferation in confined spaces occurs in biofilms, intracellular compartments, or infection sites, generating mechanical constraints. We investigated how growth-induced mechanical pressure affects bacterial physiology using a microfluidic device ensuring nutrient access. We found that proliferating Escheric...
Laure Le Blanc, Baptiste Alric, Romain Rollin et al.· Nature Communications· 0 citations
Pharmacodynamic curves describe how changes in drug concentration affect pathogen growth. They are essential for designing treatments that promote pathogen eradication and minimize the evolution of antibiotic resistance. The classical function for modelling pharmacodynamics is a phenomenological, S-shaped curve with st...
Aryan Ramachandran, Josia Pool, A. de Visser et al.· bioRxiv· 0 citations
Actin homolog MreB plays a crucial role in the synthesis of the bacterial cell wall. Disruption of the MreB filament network results in the disruption of the cell wall, which thus serves an unconventional antibacterial strategy. A22, a small inhibitor molecule, binds directly to the MreB filament, altering its structur...
E. Bódis, Beáta Longauer, A. Lukács et al.· Frontiers in Molecular Biosc...· 0 citations
Bacterial cultures entering stationary phase (SP) undergo complex physiological alterations, helping the cells to survive when medium resources are exhausted. The onset of various stress responses underlying SP physiology is generally believed to be due to reactions of individual cells to the environmental cues such as...
P. A. Ivanov, O. Timoshina, Sergey Mureev et al.· bioRxiv· 0 citations
Stopping antimicrobial multidrug resistance (AMR) is essential to maintain the efficacy of current treatments and sustain modern medicine, but current control relies on a coordinated One Health approach involving stewardship, infection prevention and other control measures, without direct actionable mechanisms against...
Hannah M. Doherty, J. S. van der Laan, D. Martínez-Martínez et al.· bioRxiv· 0 citations
NC protected Mtb from lethal doses of most standard TB drugs, and showed that disruption of the pH gradient and consequent intracellular acidification is needed to induce tolerance, and that protection is tunable by external pH.
Hassan E. Eldesouky, Kristin N. Adams, Justin K Brache et al.· mBio· 0 citations
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