A simple, quick, and reproducible protocol for reconstituting respiratory SCs into liposomes that allows for testing the effects of varying lipid compositions, protein concentration, and membrane potential on the function of respiratory SCs, providing a valuable tool for future mechanistic studies.
A. Padavannil, Pei-Yi Liu, J. Letts· Journal of Visualized Experi...· 0 citations
Mitochondrial cristae are highly curved invaginations of the inner mitochondrial membrane (IMM) that optimize oxidative phosphorylation (OXPHOS) by concentrating respiratory complexes and adenosine triphosphate (ATP) synthase. The mechanistic relationship between membrane curvature, proton motive force (PMF), and crist...
The solution structure of mature Saccharomyces cerevisiae Mba1 is determined using multidimensional nuclear magnetic resonance (NMR) spectroscopy and reveals a compact α + β fold with a central hydrophobic cavity and distinct charged surface regions, providing a structural framework for interpreting previous functional...
Jing Yang, Mao-Sen Ruan, Dong-Shuai Bai et al.· Journal of Structural Biolog...· 0 citations
Mitochondria serve as central hubs of cellular bioenergetics and signaling, yet the dynamic role of their lipid composition in cellular adaptation remains underappreciated. Unlike most organelles, mitochondria possess a unique dual-bilayer membrane architecture shaped by lipid transport and de novo synthesis. The mitoc...
M. Balážová, Li-Li Jiang, Cun-Qi Ye· ACS Chemical Biology· 0 citations
Abstract Extracellular electron transfer (EET) is a central process in the respiratory metabolism of electrogenic bacteria, enabling cells to exchange electrons with extracellular acceptors and donors. This capability has opened opportunities to exploit these bacteria in diverse practical applications, many of which wo...
Alexandre Almeida, Marta A. Silva, Jorge M. A. Antunes et al.· Biochemical Society Transact...· 0 citations
The mechanism by which glycerophospholipids are transported between the inner and outer membranes in Gram-negative bacteria remains poorly understood. In Escherichia coli, the paraquat-inducible (Pqi) pathway, comprising the inner membrane protein PqiA, the periplasm-spanning MCE-family protein PqiB, and the outer memb...
Hannah E. Johnston, Luke A. Clifton, C. Wilson et al.· bioRxiv· 0 citations
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