Efficient and precise programmable DNA knock-in without double-strand breaks

Yanmin Gao Yu Ma Ke-Xin Yu Yin-Tian Liu Buming Gu Hao Tang Wen-Jie Yan Shuang-Shuang Yang Jin-Hao Su Xin-Dong Wang Xin Ma Xin-Ming Wang Fang Wang Qingyang Li Meng-Ying Liu Haifeng Wang

Jul 2026 · Nature · Vol 657, pp. 284 - 294 · 0 citations · 83 references

Medicine

TL;DR

Kilobase-scale nickase-targeting (KNIT) editing enables single-nick-based DNA insertion across genomic loci and cell types, supporting insertion of DNA fragments exceeding 10 kb, with high efficiency and minimal unwanted off-target effects.

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