Zebrafish relatives as models for functional comparative genetics and genomics.

Comprehensive insights into molecular techniques and applications for genetic modification and targeted mutagenesis in zebrafish

This review highlights the shift from gene disruption to precise and reversible control of gene activity by combining traditional mutagenesis techniques with cutting-edge genome-editing tools and newly developed CRISPR-based regulatory systems.

Using CRISPR/Cas9 to investigate the role of candidate human disease gene orthologs in Ciona

The results identify Pgm3 as the most promising candidate for further development as a Ciona-based model of human disease and demonstrate the utility of tissue-specific CRISPR screening for prioritizing candidate disease gene orthologs identified through comparative genomics platforms like Zoogle.

Comparative Anatomy of the Central Nervous System of Danio Rerio and Mammals

Karyotypic evolution in a diverse primate clade and the role of chromosomal fissions as barriers to gene flow

The classical model of chromosomal speciation is based on the observation that divergent species often differ in chromosome numbers and that heterokaryotypic hybrids show reduced fitness. Furthermore, positive correlations between the rates of karyotype evolution and speciation have been established in several groups o...

A multiplex genome editing pipeline for rapid combinatorial trait engineering.

Current de-extinction efforts center on editing the genomes of extant species to express key traits that evolved in closely related extinct species. These complex projects require advances in genetic engineering pipelines coupled with animal model production as an approach to test hypotheses about genotype and phenotyp...

Xenbase: A Guide to the Xenopus Genetics and Genomics Knowledgebase.

Xenbase is the Xenopus frog model organism knowledgebase, an online resource ( www.xenbase.org ) that hosts fully integrated and cross-referenced genomic and biological data to support Xenopus researchers worldwide. Xenbase hosts a vast variety of gene-centric content, including annotated genomes for X. laevis and X. t...