An internal scaffold supports microtubule architectural diversity in Xenopus spermatids
TL;DR
It is established that an organized microtubule network is present in Xenopus spermatids and shares key architectural and molecular hallmarks of the mammalian manchette, and a large structural heterogeneity of spermatid microtubules is uncovered, suggesting an internal reinforcement mechanism necessary for extensive nuclear reshaping and cytoplasm remodeling.
Abstract
During spermiogenesis, early round spermatids differentiate into specialized spermatozoa through an extensive reshaping of the nucleus driven by coordinated cytoskeletal and chromatin-based mechanisms. In mammals, this process critically relies on the transient manchette, a microtubule-based structure that remodels the spermatid nucleus and serves a track to transport material required for flagellum assembly. However, the existence, organization, and molecular composition of such a structure in other vertebrates have remained poorly investigated. Here, we establish that an organized microtubule network is present in Xenopus spermatids and shares key architectural and molecular hallmarks of the mammalian manchette. We further uncover a large structural heterogeneity of spermatid microtubules, with variable protofilament numbers, skew angles, and lattice organizations. We reveal the presence of a Spaca9–Saxo2 internal scaffold in spermatid microtubules, suggesting an internal reinforcement mechanism necessary for extensive nuclear reshaping and cytoplasm remodeling.