Aug 2026· Plant Pathology Journal· Vol 42, pp. 533 - 544· 0 citations· 43 references
Medicine
TL;DR
The strong antifungal potential of Streptomyces sp.
Abstract
Fungi are major phytopathogens that have a strong impact on agricultural productivity. Recently, biological control has gained attention for managing plant pathogenic fungi due to its eco-friendly characteristics. Hence, using biological agents to replace chemical fungicides is a viable alternative approach in sustainable agriculture systems. In this study, we demonstrated the potentiality of Streptomyces sp. VNUA24 as a promising biocontrol agent. The strain strongly inhibited mycelial growth of several common pathogenic fungi. Its culture filtrate also altered fungal morphology, restricted hyphal elongation, inhibited spore germination, and suppressed fungal virulence. Biochemical assays and genomic analysis revealed the productions and encoding genes for several hydrolytic enzymes. The antiSMASH analysis identified 38 biosynthetic gene clusters in the genome. Interestingly, many of these exhibited strong homologies to clusters responsible for producing established antifungal metabolites such as ɛ-poly-L-lysine, concanamycin A, informatipeptin, and humidimycin. These findings highlight the strong antifungal potential of Streptomyces sp. VNUA24 and suggest that it is a promising candidate for developing microbial control agents in sustainable fungal disease management.
Plant pathogenic fungi pose a major threat to global crop production and food security, necessitating the development of sustainable control agents. This study investigated the antifungal potential of Pseudophaeolus soloniensis, a wood-decaying fungus. A wild strain was isolated from Hebei, China, and identified via morphology and ITS sequencing. A bioactive crude extract (P1) was obtained through optimized solid-state fermentation on A3M medium. Its antimicrobial spectrum was evaluated against major phytopathogenic fungi (e.g., Fusarium graminearum, Aspergillus flavus) and model bacteria (Staphylococcus aureus, Escherichia coli) using mycelial growth inhibition, Minimum Inhibitory Concentration (MIC), and agar diffusion assays. P1 exhibited strong, selective activity, showing significantly greater inhibition against S. aureus than E. coli and pronounced effects against F. graminearum (43.79% inhibition at 20 µg/mL) and A. flavus (73.5% at 0.2 mg/mL). A hormetic-like response was observed for F. oxysporum. Liquid Chromatography-Mass Spectrometry (LC-MS) analysis revealed a diverse secondary metabolome, including flavonoids, alkaloids, quinones, and saponins. These results establish P. soloniensis as a promising source of bioactive metabolites for developing eco-friendly fungicides.
Chang Liu, Xiao-Xue Zhao, Heng Zhao et al.· Journal of Fungi· 0 citations
Endophytic bacteria represent a promising, sustainable alternative to synthetic agrochemicals for plant disease management by actively antagonizing phytopathogens and stimulating host immune responses. Despite its traditional use as an antimicrobial remedy, Wedelia chinensis remains underexplored as a source of beneficial endophytes. This study aimed to isolate and screen endophytic bacteria from W. chinensis for their antifungal activity against Fusarium oxysporum. Among 14 endophytic isolates, Bacillus subtilis LS1-5 showed the strongest antifungal activity by dual culture assay. Its cell-free culture filtrate effectively inhibited fungal spore germination by 91.3% at a 20% concentration. Crucially, B. subtilis LS1-5 produced several extracellular enzymes, including cellulase, amylase, chitinase, pectinase, gelatinase, lipase, and protease. Furthermore, the LS1-5 strain exhibited a broad array of plant growth-promoting and environmental resilience traits. Notably, it synthesized indole-3-acetic acid (IAA) at 2.11 ± 0.27 µg/mL in an LB medium without L-tryptophan, significantly promoted a 34.19% increase in root elongation, and demonstrated remarkable tolerance to extreme conditions, including high salinity (11% NaCl) and broad pH levels ranging from 6 to 12. Additionally, volatile organic compounds (VOCs) emitted by LS1-5 significantly inhibited the severe bacterial pathogens Xanthomonas oryzae (45.83%) and Xanthomonas citri (47.99%). These findings highlight B. subtilis LS1-5 as a highly effective candidate for biological control and sustainable agricultural applications.
D. Nguyen, Dong Tang Vien, Ngoc-Minh Truong Bui et al.· IOP Conference Series: Earth...· 0 citations
Plant diseases caused by phytopathogenic fungi lead to significant economic losses in agriculture. Bacteria isolated from compost have been shown to exhibit suppressive activity against these pathogens. The aim of this study was to isolate bacteria from five compost samples differing in origin and physicochemical properties and to evaluate their biocontrol potential. A total of 38 bacterial isolates were obtained, of which 12 were selected for further characterization. These isolates were subjected to morphological and biochemical analyses, identified using MALDI-TOF MS, and tested for their ability to produce siderophores and inhibit selected phytopathogenic fungi (Botrytis cinerea, Penicillium olsonii, Fusarium oxysporum, and Alternaria alternata). Eight isolates demonstrated the ability to produce siderophores. The highest production was observed in isolate 68A-4 (H/G = 44.98 ± 9.66) and Brevundimonas diminuta 512B-6 (H/G = 23.36 ± 2.34). Antifungal assays revealed variable inhibitory effects on mycelial growth. The isolate Serratia sp. 19A-5 exhibited the strongest antifungal activity and showed no statistically significant difference compared to the reference strain Bacillus subtilis QST 713 in inhibiting Penicillium olsonii and Fusarium oxysporum. These results suggest that Serratia sp. 19A-5 represents a promising candidate for compost fortification with enhanced antifungal properties. The use of such beneficial bacteria as biological control agents represents a sustainable alternative to chemical pesticides, contributing to reduced environmental impact.
J. Maková, S. Javoreková, Kristína Zuzčinová et al.· Journal of Microbiology, Bio...· 0 citations
Overall, these results confirm that B. amyloliquefaciens EG6.1, in its new formulation consisting of pellet fractions and sonicated culture, shows great potential as a green biocide and contributes to the development of disease management strategies, while supporting SDG’s 2 and 12.
F. A. Nafidiastri, Salamun, A. Supriyanto et al.· Brazilian Journal of Biology· 0 citations
The integrated experimental and in silico approach highlights soil-derived actinomycetes as versatile and sustainable bioresources with significant pharmaceutical and biotechnological potential, emphasizing their role in combating antimicrobial resistance and enabling the rational development of novel therapeutic and industrial products.
Meghana Arivilu, Shaziya Sulthana, Vijay Ramesh et al.· Journal of Pure and Applied...· 0 citations
