TL;DR
This chapter introduces the model "membrane army" arranged in a dimple chip to study the delivery of β-galactosidase by a CPP known as Pep-1 and accelerates the speed to screen through the working conditions in CPP-assisted protein translocations.
The study underscores the importance of membrane engineering as a powerful strategy for advancing the rational development of artificial cells with functional capabilities by identifying the limiting factor that underlies the observed differences in protein expression between membrane formulations: the efficiency of ma...
Membrane tension, defined as the energy cost to expand the membrane surface area, is a fundamental mechanical property of the lipid bilayer and an important modulator of various cellular processes. Liposomes provide model systems to study protein-mediated membrane dynamics, where membrane tension can be controlled by o...
Proteins and lipids of a biological cell work together to reshape the membranes in which they reside. Membrane reshaping is necessary for vesicular traffic and maintenance of organelle shape and structure. Curvature is the fundamental mathematical description of the local shape of a surface. Through the well-establishe...
Lipid membrane permeability is fundamental for cellular homeostasis, signaling, and drug transport. Experimental methods exist to characterize it, from cell-based assays to more reproducible artificial, filter-supported systems. Although they closely mimic physiological environments, these assays pose challenges for qu...
This study uses giant unilamellar vesicles mimicking the endosomal membrane to directly visualize the interaction and membrane disruptions caused by two model LNPs constituted by two different ionizable lipids, establishing a cell-free platform for dissecting individual sub-steps of endosomal escape.
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