AI Networking Cookbook: Practical recipes for AI-assisted network automation and development
TL;DR
It is suggested that the engineered H3 tail does not confer a general functional advantage within the KRAB–DNMT3L–dCas9 architecture under the conditions tested.
In animals, plants, and some fungi, Polycomb Repressive Complex 2 (PRC2) catalyzes trimethylation of histone H3 lysine 27 (H3K27me3) to establish transcriptionally repressed chromatin. Here, we identify the histone acetyltransferase RTT109 as a key regulator of PRC2-repressed domains in the model fungus Neurospora cras...
A compact and programmable epigenome editor can be utilized for long-term gene silencing, which becomes a powerful tool for biological research or biomedical applications. Here, we present an enhanced CRISPR-based epigenome editor based on deactivated AsCas12f1 (dAsCas12f1) variants, which incorporate epigenetic modula...
Histone H3 lysine 36 dimethylation (H3K36me2) plays a key role in transcriptional elongation and chromatin organization, yet how its deposition is spatially controlled across the genome remains unclear. Here, we identify TOP1 as a critical recruiter of the H3K36me2 methyltransferase NSD2 to gene bodies. Disruption of t...
DNA replication dilutes parental histones and their post-translational modifications, requiring restoration of epigenetic marks on newly incorporated histones. H3K9me3 restoration is unusually slow, extending into the following G1 phase, but the mechanisms governing this delay remain unclear. Here, we show that RIF1 re...
Histone post-translational modifications (PTMs) segregate genomes into heterochromatin and euchromatin by recruiting readers, such as Heterochromatin Protein 1, which binds methylation of histone H3 lysine 9 (H3K9me). Here, we report H3K9me-independent functions for the C. elegans HP1 orthologue HPL-2 and the H3K9 meth...
Spermatogenesis begins with the gonocyte-to-spermatogonia transition (GST) during the perinatal window, yet how chromatin regulation orchestrates this developmental transition remains elusive. Transcriptomic analysis identified histone acetylation as a significantly enriched pathway during the GST, and immunofluorescen...
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