Molecular Cloning and Functional Analysis of CTL14 and PPO4: Their Roles in Development, Reproduction, and Antiviral Defense in Hyphantria cunea
TL;DR
Findings underscore the crucial roles of CTL14 and PPO4 as they are involved in both the antiviral defense system and the physiological development of H. cunea, thereby identifying them as potential novel targets for biological pest control strategies.
Abstract
Simple Summary The fall webworm (Hyphantria cunea) is a destructive invasive pest that causes serious damage to forest and agricultural plants. Understanding its immune system is important for developing effective and environmentally friendly control strategies. In this study, we investigated the roles of two immune-related genes, CTL14 and PPO4, in the larval development, antiviral defense, and reproduction of H. cunea. Silencing these genes via RNA interference significantly reduced larval survival, growth, and resistance to nucleopolyhedrovirus infection. Moreover, gene silencing led to severe reductions in adult fecundity and egg hatchability, especially after CTL14 suppression. Conversely, injection of purified immune proteins improved larval fitness and growth efficiency. These findings demonstrate that CTL14 and PPO4 are essential for both immunity and development in H. cunea and highlight their potential as molecular targets for sustainable pest control strategies.