Development of iFCCS for Nanometer Sizing of Transmembrane Proteins in GUVs

Curvature sensing and generation by diverse membrane-tethered polymers can be explained by a unifying physical model

Characterization of Single Ribosomes and Virus Particles with Large-Diameter Perfringolysin O Nanopores

The self-assembly of the cholesterol-dependent cytolysin Perfringolysin O (PFO) into stable transmembrane nanopores composed of 46 ± 9 monomers is reported, enabling, for the first time, resistive-pulse-based characterization of intact virus particles and ribosomes with a biological nanopore.

A Biophysical Hypothesis for Compartment-Specific Conformational States of the IQSEC2 Intrinsically Disordered Protein.

It is proposed that, due to fundamental biophysical differences between the dendritic cytosol and the postsynaptic density, there are two distinct classes of IDPs functioning in these different environments and these differences should be considered when optimizing force fields and parameters of water models for studyi...

Giant Vesicle Platform for Synthetic Endoplasmic Reticulum Networks

The endoplasmic reticulum (ER) is a membrane-bound organelle found in all eukaryotic cells. This organelle has a fascinating morphology, which involves a network of membrane nanotubes and three-way junctions connecting three nanotubes. Previous reconstitution studies were based on proteo-liposomes with a size of 100 to...

Studying the Protein-Membrane Interaction of a Mycobacterial ABC Transporter Through Coarse-Grained Simulations.

An Automated HDX−MS Platform for Characterization of Membrane Proteins within Biological Membranes

This work presents the first fully automated HDX−MS platform incorporating a two-stage delipidation workflow and underscores the importance of studying membrane proteins in native lipid environments.