Jul 2026· Applied Biochemistry and Biotechnology· 0 citations· 40 references
Medicine
TL;DR
This work establishes a generalizable and scalable platform for the biomanufacturing of cereal-derived bioactive peptides, providing a practical alternative to conventional methods and enabling future studies on peptide structure-function relationships and industrial applications.
It is demonstrated that maximizing recombinant protein production requires optimization of both yield and structural quality and establish complementary strategies for improving secreted protein expression in Pichia.
This review summarizes recent advances in engineering key expression elements underlying heterologous protein production in K. phaffii, with particular emphasis on promoter architecture redesign, signal peptide replacement and sequence engineering, molecular chaperone co-expression, and quantitative regulation of the u...
Ru-Yue Han, Ruizheng Hu, An-Ran Liu et al.· Journal of Fungi· 1 citation
Milk proteins deliver nutritional, functional, and bioactive properties that alternative protein sources cannot adequately replicate, yet conventional livestock-based production faces compounding constraints of scalability, resource intensity, and sustainability. Precision fermentation offers a structurally distinct so...
Zhengtao Guo, Kun Ye, Zhen-Ye Shi et al.· Comprehensive Reviews in Foo...· 0 citations
Results show that efficient PGG production in B. subtilis requires coordinated control of secretion, extracellular stability and proenzyme activation, and a standardized modular Golden Gate-based secretion platform in Bacillus subtilis is developed to screen promoter-signal peptide combinations for extracellular produc...
Clàudia Lliso-Pascual, Sergi Abad, Marc Carnicer et al.· Applied Microbiology and Bio...· 0 citations
A refolding-free bioprocess for high-yield soluble expression of B2036 in E. coli using SUMO fusion provides a valuable reference for developing efficient soluble expression and downstream purification processes of other intrachain disulfide-bonded proteins in E. coli.
Haoju Hua, Yapeng Wang, Jun Feng et al.· Bioprocess and biosystems en...· 0 citations
Soy-derived small peptides (SSPs, 2–5 amino acids) are food-derived bioactive peptides with efficient intestinal absorption, primarily via peptide transporter 1 (PepT1), and greater digestive stability than longer peptides. This review systematically summarizes 175 SSPs reported between 1996 and 2026, covering their...
Mubeen Asad, Zhen Wang, Ahsan Javed et al.· Journal of Agricultural and...· 0 citations
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