The near-complete backbone and partial side-chain NMR resonance assignments of the C-terminal domain of human 5MP1, carrying a W404E substitution that disrupts dimerization are reported, providing a foundation for future investigations of the molecular interactions, dynamics, and functions of 5MP1 in translation initiation.
This work establishes that multisite phosphorylation of the SPT5 CTR1 domain modulates a heterogeneous interaction landscape shaped by sequence context, and emphasizes the nontrivial difference between serine and threonine phosphorylation as a mechanism to regulate disordered protein structure–function relationships.
Rong Hu, E. Usher, Olivia A Fraser et al.· Journal of Physical Chemistr...· 0 citations
Full‐length atomistic conformational ensembles generated by IDPConformerGenerator and optimized by X‐EISDv2 workflow support a model of translation regulation in which the dynamic 4E‐BP2:eIF4E complex facilitates accessibility of regulatory sites of 4E‐BP2 when bound.
S. Smyth, Zi-Hao Liu, Thomas E. Tsangaris et al.· Protein Science· 0 citations
Findings establish m1A58 as a conformational checkpoint coupling human initiator-tRNA maturation to translation initiation and stress responses, and establish m1A58 as a conformational checkpoint coupling human initiator-tRNA maturation to translation initiation and stress responses.
Hao Li, Xing Wu, You Zhou et al.· bioRxiv· 0 citations
Abstract Aminoacyl-tRNA synthetases (AARSs) safeguard translational fidelity by coordinating amino acid activation and tRNA charging within distinct catalytic and editing domains. In leucyl-tRNA synthetase (LeuRS), the small, centrally located zinc-binding domain (ZN domain) sits at the crossroads of these functional c...
G. Hoffmann, Morana Dulic, I. Gruic‐Sovulj et al.· Nucleic Acids Research· 0 citations
A mammalian fluorescence resonance energy transfer (FRET) based high-throughput reporter system is developed to quantitatively measure 2A activity across large libraries of natural and engineered 2A peptides and provides mechanistic and evolutionary insights into how nascent peptide–ribosome exit tunnel interactions me...
Adesh Baral, Aditi Kothiala, K. X. Zhong et al.· bioRxiv· 0 citations
It is concluded that the early emergence of the Rossmann fold reflects the chemical and physical constraints of protein folding, explaining both its profound antiquity and sustained longevity.
Koh Seya, Tatsuya Corlett, Hamza Giaffar et al.· bioRxiv· 0 citations
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