cerevisiae-based platform for rapid production and evaluation of Eukaryotic Nutrient transporters and transceptors for biochemical studies and crystallography
A new effective S. cerevisiae expression system is developed that may be used for production of high-quality eukaryotic membrane proteins for functional and structural analysis.
A case-study evaluation of a pBR322-derived expression system, previously applied to therapeutic peptides and insulin analogs, in combination with fed-batch cultivation for recombinant production of methionine aminopeptidase in E. coli, evaluating the performance of this system for methionine aminopeptidase as a stress...
G. Kuznetsov, Marina Yarovikova, E. Buslaeva et al.· Protein Expression and Purif...· 0 citations
This review summarizes recent advances in engineering key expression elements underlying heterologous protein production in K. phaffii, with particular emphasis on promoter architecture redesign, signal peptide replacement and sequence engineering, molecular chaperone co-expression, and quantitative regulation of the u...
Ru-Yue Han, Ruizheng Hu, An-Ran Liu et al.· Journal of Fungi· 1 citation
A generalizable workflow for producing soluble receptor domains from membrane proteins is established and validated HasR constructs suitable for downstream ligand-screening applications, including aptamer and nanobody discovery.
Federico Bosetto, Maria Zacharopoulou, M. Bycroft et al.· bioRxiv· 0 citations
This work developed a Green Fluorescent Protein (GFP) secretion system by fusing GFP of the N-terminus sequence from the curli monomer protein while co-expressing the curli export machinery and found that secretion was sequence-specific, although no simple metric could predict success.
Anton Kan, S. Emani, Neel S. Joshi· ACS Synthetic Biology· 0 citations
It is demonstrated that maximizing recombinant protein production requires optimization of both yield and structural quality and establish complementary strategies for improving secreted protein expression in Pichia.
Results show that efficient PGG production in B. subtilis requires coordinated control of secretion, extracellular stability and proenzyme activation, and a standardized modular Golden Gate-based secretion platform in Bacillus subtilis is developed to screen promoter-signal peptide combinations for extracellular produc...
Clàudia Lliso-Pascual, Sergi Abad, Marc Carnicer et al.· Applied Microbiology and Bio...· 0 citations
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