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Efficient exploration of sequence space enables rapid generation of functional genome editors

Nicholas W. Hughes Sourab Kulkarni Grant Goldman Julia Marsiglia Sunit Jain Kaitlyn K. Spees Becky Xu Hua Fu Kia Vaalavirta Muneaki Nakamura

Aug 2026 · bioRxiv · 0 citations · 21 references

Biology

TL;DR

An approach to create novel, functional proteins through the integration of deep mutational scanning, structural analysis, and evolutionary mining within prompts for a generative protein language model (PLM) is described and the utility of this approach is demonstrated with the generation of novel compact RNA-guided nucleases.

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