An integrated signal amplification strategy based on catalytic hairpin assembly and hybridization chain reaction for driving a CRISPR/Cas12a biosensor toward ultrasensitive detection of microRNAs

Programmable hairpin assembly circuit-cascaded DNAzyme amplification for ultrasensitive miRNA-146a detection via high-stable perovskite nanocomposite biosensor.

Hairpin-Structured DNAzyme-Initiated Spherical Hybridization Chain Reaction Cascade Enables Highly Sensitive Electrochemical Detection of MicroRNA

A homogeneous cascade amplification-based electrochemical biosensing platform that integrates hairpin-structured DNAzyme-assisted target recycling amplification with spherical hybridization chain reaction (SHCR)-enhanced electrochemical signal transduction, enabling highly sensitive detection of miRNA-141 (miR-141).

Catalytic Hairpin Assembly-Aided CRISPR/Cas-SERS for Nucleic Acid Detection Enhanced by Multiple Catalytic Amplification

Owing to the collateral trans-cleavage activity of Cas12a and the high peroxidase-like nanozyme activity of G4/Hemin, this method achieves the highly sensitive SERS detection of genetically modified samples without target gene preamplification.

An RCA-Coupled CRISPR/Cas12a Cascade-Enhanced SERS Platform for Ultrasensitive Detection of Exosomal miRNA-21 in Colorectal Cancer Serum

Purpose Ultra-sensitive detection of exosomal miR-21 is crucial for the early diagnosis of colorectal cancer. This study aims to develop a cascade amplification platform based on surface-enhanced Raman scattering (SERS), combining rolling circle amplification (RCA) and CRISPR/Cas12a, to enable ultra-sensitive quantitat...

A CRISPR/Cas9 multiplex nucleic acid sensing platform based on rolling circle amplification-mediated monomeric substrate generation.

The multiplex detection of nucleic acid biomarkers, including circulating tumor DNAs (ctDNAs) and microRNAs (miRNAs), holds promise for improving the accuracy of molecular diagnostic techniques. In this study, we developed a CRISPR/Cas9-based sensing platform capable of simultaneously detecting multiple nucleic acid bi...

Engineering a leakage-resistant CHA-CRISPR/Cas12a biosensor via cis-cleavage-triggered split activator assembly for ultrasensitive miRNA detection.

MicroRNAs (miRNAs) have emerged as important biomarkers for cancer diagnosis and prognosis, yet their accurate detection remains challenging due to low abundance, short sequences, and sequence homology. Herein, we report a novel biosensing platform based on a cascade cis-cleavage-triggered split activator assembly stra...