Aug 2026· Proceedings of the National Academy of Sciences of the United States of America· Vol 123· 0 citations· 86 references
Medicine
TL;DR
The mutant POLD1 enzyme reported here introduces mutations into the viral genome during replication, thereby disrupting the function of essential viral proteins, and is a powerful resistance mechanism that plant geminiviruses cannot escape.
Abstract
Significance The cultivated tomato is the most widely grown vegetable crop worldwide, but its production is severely impacted by geminivirus infections. Therefore, finding robust resistance genes against these infections would be of significant economic benefit. Recently, mutations in DNA POLYMERASE DELTA 1 (POLD1) have been associated with resistance to geminivirus infections. However, the mechanism by which these mutations confer resistance remains unknown. In tomato, mutant POLD1 alleles are located at the Ty-6 resistance locus introgressed from the wild tomato Solanum chilense. The mutant POLD1 enzyme reported here introduces mutations into the viral genome during replication, thereby disrupting the function of essential viral proteins. Currently, the mutant POLD1-mediated inactivation of geminiviruses is a powerful resistance mechanism that plant geminiviruses cannot escape.
The present study represents the first application of base editing in barley plants, using the EIF4E gene as an example, and two newly generated alleles led to resistance upon BaMMV inoculation without adverse effects on yield, proving this approach promising to generate new material for resistance breeding.
R. Hoffie, A. Habekuss, D. Perović et al.· bioRxiv· 0 citations
This study demonstrates that expression of tomato leaf curl New Delhi virus CP confers effective resistance against tomato yellow leaf curl China virus (TYLCCNV), another prominent geminivirus, and uncovers a novel mechanism by which a viral effector antagonizes viral CP-mediated host resistance during mixed viral infe...
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C. Bianco, Alex C. Stabell, Manivel Lodha et al.· bioRxiv· 0 citations
The transcriptomic and genomic changes in the two virulent M. incognita isolates investigated highlight genome structural plasticity as one of the potential key drivers of virulence acquisition in these asexually reproducing parasites.
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