Overall, CRISPR-Cas-based genome editing represents a promising and efficient approach for accelerating the development of high-yielding, climate-resilient, and stress-tolerant rice cultivars, thereby contributing significantly to sustainable rice production and global food security under changing environmental conditions.
This review systematically examines how CRISPR-Cas9 enables targeted engineering of stress tolerance in major crops through gene knockout and knock-in strategies, and highlights emerging synergies with functional genomics, multi-omics integration, and high-throughput phenotyping to accelerate target discovery and validation.
This review critically synthesizes recent advances in CRISPR applications for major wheat fungal diseases, including powdery mildew, rusts, Fusarium head blight, and wheat blast, and highlights future opportunities for integrating genome editing with modern breeding to accelerate the development of climate-resilient, disease-resistant wheat cultivars for sustainable agriculture.
The regulatory networks of G protein subunits in yield and quality traits and stress responses are summarized, the mechanisms underlying G protein‐mediated growth‐resistance decoupling are dissected, and precision strategies to simultaneously enhance these agronomic traits are proposed.
This review synthesises the fundamental principles and limitations of multiple gene-editing technologies, with a particular emphasis on CRISPR systems (Cas9, Cas12, and Cas13), in the specific context of woody perennial biology, and highlights how synergising CRISPR technologies with multi-omics, genomic selection, and high-throughput phenomics can accelerate the development and application of climate-resilient woody perennials.
Climate change increasingly threatens global crop productivity by intensifying drought, salinity, temperature extremes, and biotic stresses. Developing climate-resilient cultivars has therefore become a central objective in modern crop breeding programs. Conventional breeding approaches are often limited by complex trait inheritance and long selection cycles, particularly for polygenic stress-adaptive traits. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated protein (Cas) genome editing genome editing provides a precise and efficient platform for targeted manipulation of genes controlling stress tolerance, yield stability, and adaptive performance. This review synthesizes recent advances in CRISPR mediated improvement of resilience to major abiotic stresses (drought, salinity, heat, and cold) and biotic stresses (fungi, bacteria, viruses, and insects) across important cereal, legume, and horticultural crops. Emphasis is placed on the editing of transcription factors, signaling regulators, susceptibility genes, and redox-associated pathways that enhance physiological and molecular stress adaptation. Furthermore, the integration of CRISPR with genomics, transcriptomics, proteomics, metabolomics, genome-wide association studies, high-throughput phenotyping, and artificial intelligence-driven prediction tools is accelerating precision breeding strategies. Despite remaining challenges related to off-target effects, delivery systems, and regulatory frameworks, genome editing represents a transformative approach for advancing climate-resilient crop development and sustainable agricultural production.
