Biophysics and Physicobiology

Membrane Formulation Impacts Vesicle-Based Artificial Cell Functions

The study underscores the importance of membrane engineering as a powerful strategy for advancing the rational development of artificial cells with functional capabilities by identifying the limiting factor that underlies the observed differences in protein expression between membrane formulations: the efficiency of ma...

Lipid nanoparticle induced model endosomal membrane disruption

This study uses giant unilamellar vesicles mimicking the endosomal membrane to directly visualize the interaction and membrane disruptions caused by two model LNPs constituted by two different ionizable lipids, establishing a cell-free platform for dissecting individual sub-steps of endosomal escape.

Paraformaldehyde-Induced Vesiculation Preserves Native Membrane Physiological States Critical for T‑Cell Activation

Plasma membrane vesicles (PMVs) are powerful model systems for studying plasma membrane biophysics, but their production typically relies on chemicals, such as dithiothreitol (DTT) and paraformaldehyde (PFA). DTT is detrimental to protein structure and can alter membrane composition, whereas PFA is a well-established f...

Modeling Lipid Vesicles’ Dynamic Response to Osmotic Pressure to Quantify Small-Molecule Transport

Lipid membrane permeability is fundamental for cellular homeostasis, signaling, and drug transport. Experimental methods exist to characterize it, from cell-based assays to more reproducible artificial, filter-supported systems. Although they closely mimic physiological environments, these assays pose challenges for qu...

Bottom-up engineering of EV-mimetic vesicles reveals selective protein loading and modulates cellular uptake and biodistribution

Regulating the membrane-fusion effect of liposomes via the protonation of tertiary amines.

It is demonstrated that PsFul enables direct cytosolic delivery of anticancer drugs via membrane fusion, bypassing lysosomal entrapment and significantly enhancing cytotoxicity under acidic conditions.