Development of A New Spectrophotometric Determination of Ceftriaxone Based on The Oxidative Coupling Reaction with N, N-diethyl-p-phenylenediamine
TL;DR
The proposed method based on an oxidative coupling reaction with the reagent N,N-diethyl-p-phenylenediamine, using sodium periodate as the oxidizing agent in an acidic medium indicates that the proposed method provides a simple, reliable, and sensitive analytical tool for the routine analysis of pharmaceutical preparations.
Abstract
In this study, a simple and sensitive spectrophotometric method was developed for the determination of ceftriaxone based on an oxidative coupling reaction with the reagent N,N-diethyl-p-phenylenediamine, using sodium periodate as the oxidizing agent in an acidic medium. The reaction produced a red-colored compound with a maximum absorption at 562 nm. The method showed good linearity within the concentration range of 6–60 µg/mL, characterized by a high correlation coefficient (r = 0.9999) and a molar absorptivity of 6488.58 liters · mol⁻¹·cm⁻¹, whilst the limit of detection (LOD) and limit of quantification (LOQ) were 0.37 and 1.11 µg/mL, respectively. The method also demonstrated good precision and reproducibility, with a relative standard deviation (RSD) not exceeding 0.57 percent, and the results of the interference and standard-addition studies confirmed its suitability for determining ceftriaxone in pharmaceutical preparations. The results indicate that the proposed method provides a simple, reliable, and sensitive analytical tool for the routine analysis of pharmaceutical preparations.