Characterization of Streptomyces virginiae JCK-8401 for the Suppression of Tomato Bacterial Wilt.

Abstract

Bacterial wilt caused by Ralstonia solanacearum is a destructive soil-borne disease affecting numerous crops worldwide. Although synthetic pesticides and antibiotics are commonly used for disease management, their excessive application has raised concerns regarding environmental pollution, human health risks, and the emergence of antibiotic-resistant pathogens. As a sustainable alternative, rhizosphere microorganisms have gained attention as potential biocontrol agents. In our previous study, strain JCK-8401 exhibited strong antibacterial activity against R. solanacearum. In the present study, its biocontrol potential was comprehensively characterized through analyses of physiological and biochemical traits, antagonistic activity against phytopathogens, induction of salicylic acid (SA)-mediated defense responses using Arabidopsis thaliana PR1::GUS reporter line, and in vivo suppression of tomato bacterial wilt. Based on 16S rRNA sequence analysis, JCK-8401 was identified as Streptomyces virginiae. The strain utilized diverse carbon sources and produced hydrolytic enzymes and indole-3-acetic acid. Furthermore, it exhibited antibacterial activity against various phytopathogenic bacteria and oomycetes, though it lacked antifungal activity. Both cell suspension and culture filtrate of the strain induced GUS expression, indicating activation of the SA-mediated defense pathway. In vivo assay demonstrated that seed treatment with JCK-8401 achieved 96.40% control efficacy against tomato bacterial wilt. Additionally, pretreatment with diluted culture filtrates (250-, 500-, and 1000-fold) effectively suppressed disease incidence regardless of the application method, strongly suggesting the activation of induced systemic resistance. Overall, Streptomyces virginiae JCK-8401 is a highly promising, multifunctional biocontrol agent for managing soil-borne diseases through a synergistic combination of antibiosis, bioinoculation, and the induction of host plant defense responses.