The data demonstrate that MRO is poised to play a role in the coordination of periovulatory events through the EGF and progesterone signaling pathways through the EGF and progesterone pathways.
Abstract
Maestro (MRO) is a transcription factor that has a role in regulating gene expression. The MRO expression pattern was examined in a unique set of human granulosa cells (GCs) and follicles collected across the ovulatory period of a natural menstrual cycle. MRO mRNA was elevated (70-fold) in women during the early ovulatory phase (12h to ≤18h post hCG administration; LH analog) and remained elevated (35-fold) in the late ovulatory phase (>18h to ≤ 34h) when compared to the preovulatory state (before the LH surge). Immunohistochemistry of whole follicles from women demonstrated positive MRO signal in GCs during the early and late ovulatory phases. Employing a cultured human granulosa luteal cell (hGLCs) model, we further examined MRO regulation in human granulosa cells from IVF patients. Treating hGLCs in vitro with hCG elevated MRO expression by 22-fold at 6h, 48-fold at 12h, and then expression slightly decreased to 13-fold at 24h. Similarly, the strongest MRO protein expression was detected at 12h. Next, LH/hCG signaling pathways regulating MRO expression were investigated using inhibitors specific to each signaling pathway. Our data revealed that hCG regulated MRO expression through LH-dependent classical signaling pathways such as PKA, PKC, and PI3K, with MAPK partially regulating its expression. Moreover, the EGF and progesterone pathways, important in the periovulatory period, were found to be involved in MRO regulation. These data, taken together, demonstrate that MRO is poised to play a role in the coordination of periovulatory events through the EGF and progesterone signaling pathways.
This study provides the first comprehensive temporal transcriptomic atlas of the donkey endometrium, revealing conserved endocrine–immune–metabolic regulatory networks comparable to those in mares and cattle.
Findings indicate that adropin may regulate porcine follicular development through its actions on Gc and oocytes through its actions on gonadotropin and steroid treatment.
Patrycja Kurowska, Alicja Łapa, Jakub Chatys et al.· Biology of Reproduction· 0 citations
Roe deer (Capreolus capreolus) are seasonal animals with fertilisation taking place in summer (July/August). Thereafter, the embryo enters a 4-5-month period of embryonic diapause. This reproductive strategy, displaying a reduced embryonic developmental velocity at the blastocyst stage, leads to reactivation and implantation in late December-January and parturition in spring (May/June). The endocrine mechanisms governing reactivation, implantation and placentation remain poorly understood. Here, we applied ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) to profile circulating progestogens from reactivation through late gestation (December-April) and in placental and luteal tissue. Notably, the neurosteroid pregnanolone (3α,5β-THP) appeared exclusively in pregnant does from late December/early January onwards, coinciding with the period of implantation. Pregnanolone was detected in placental but not luteal tissue, suggesting the placenta as a potential source of origin. Our results indicate a previously unrecognised shift in progestogens from reactivation/early placentation onwards, suggesting that non-classical progestogens may contribute to the endocrine regulation of implantation and pregnancy maintenance. Further investigation into the origin and functional roles of pregnanolone are needed to elucidate its contribution to pregnancy and reproductive success in roe deer.
Sara Elsafadi, Haolin Chen, R. Giacometti et al.· Reproduction· 0 citations
This study provides the first single-cell transcriptomic atlas of goat ovarian development, delineating dynamic gene expression patterns and cellular interactions during primordial follicle pool establishment, and advance understanding of conserved and species-specific mechanisms governing female reproductive lifespan in mammals.
Yong Ruan, Dongwei An, Lingang Dai et al.· Reproduction· 0 citations
The unique spatial confinement and regulatory capacity of perifollicular SCs endow them with the potential to become important components of the follicular functional unit, providing new theoretical support for understanding the molecular regulatory mechanisms of ovarian aging from the perspective of the follicular microenvironment.
Zhe Zhang, Na Kong, J. Mei et al.· Cell Discovery· 0 citations
Reconstructing a map of intrafollicular interactions provides novel insights and important clues for understanding cellular and molecular mechanisms that fine-tune antral folliculogenesis and offers a roadmap for predicting candidate factors that can be used for optimizing mammalian in vitro embryo production systems.
Zhaochen Wang, Zhenni Zhang, Ya-Wen Tang et al.· Journal of Animal Science an...· 0 citations
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