Aug 2026· Insects· Vol 17· 0 citations· 37 references
Medicine
TL;DR
Findings indicate that LmRab11A functions as an important regulatory factor influencing gene expression, metabolic homeostasis, and developmental processes in Locusta migratoria, thereby contributing to normal growth and molting.
Abstract
Simple Summary In our previous study, we found that LmRab11A plays a critical role in the molting process of the migratory locust. To further investigate the function of LmRab11A, in this study, we employed RNA interference (RNAi) to silence the LmRab11A gene, followed by integrated transcriptomic and metabolomic analyses. Transcriptomic analysis combined with RT-qPCR revealed that 9 genes were significantly downregulated following LmRab11A knockdown. Subsequent RNAi-mediated knockdown of these 9 genes showed that silencing only LOCMI11062 (β-tubulin) led to arrested molting and 100% mortality in locust nymphs, a phenotype reminiscent of that caused by LmRab11A knockdown, indicating the essential role of β-tubulin in development. Metabolomic analysis identified 11 significantly altered metabolites, 8 exhibiting decreased abundance and 3 exhibiting increased abundance. Spearman correlation analysis between the 9 downregulated genes and the metabolite suggested potential associations between 6 metabolites and specific genes. Taken together, these findings indicate that LmRab11A functions as an important regulatory factor influencing gene expression, metabolic homeostasis, and developmental processes in Locusta migratoria, thereby contributing to normal growth and molting. This study expands our understanding of the molecular mechanisms underlying LmRab11A function and identifies potential targets for the development of novel locust management strategies.
Nesfatin-1 has biological roles including the suppression of food intake and the regulation of glucose and lipid metabolisms. However, th8e information available regarding nesfatin-1 in the glycolipid metabolism in the early development stage of fish is still limited. In order to investigate the role of the nesfatin-1 gene in the early development stage of the largemouth bass (Micropterus salmoides), the nesfatin-1 gene was inhibited using siRNA interference technology. Then, we evaluated their mRNA expression levels, transcriptomes and metabolomes. The mRNA expression levels of nesfatin-1 gene were appreciably decreased at 48 h,72 h and 96 h after injection of nesfatin-1 siRNA in the early development stage. The omics results revealed that the nesfatin-1 gene was interfered to induce 1833 differentially expressed genes (DEGs) and 2370 differentially expressed metabolites (DEMs). Bioinformatic analysis enriched the most affected molecular pathways (sphingolipid metabolism, fatty acid elongation, amino sugar and nucleotide sugar metabolism and biosynthesis of unsaturated fatty acids) and metabolic pathways (biosynthesis of unsaturated fatty acids, sphingolipid metabolism and amino sugar and nucleotide sugar metabolism) in early development of largemouth bass. In amino sugar and nucleotide sugar metabolism, increased expression levels of genes such as chic, chs1, and gck genes, alongside decreased expression levels of the chia.1 gene, resulted in significantly elevated concentrations of N-Acetyl-D-glucosamine, beta-d-Fructose 6-phosphate, beta-d-Fructose, D-Mannose 6-phosphate, d-Glucose, d-Glucose 1-phosphate, UDP-glucose, UDP-glucuronate, whilst the concentration of UDP-N-acetyl-alpha-D-glucosamine was markedly reduced. Therefore, the nesfatin-1 gene could influence the early development stage of largemouth bass by affecting signaling pathways associated with glycolipid metabolism. Our findings further expand the molecular mechanisms of nesfatin-1 gene, and provide further theoretical support for the initial breeding and feed adaptation of largemouth bass.
Shiping Su, Xin-Xin Liu, Junqing Li et al.· Genomics· 0 citations
Lipids are vital cellular components with structural, storage, signaling, and defensive functions, and lipid metabolism is critical to strawberry quality. However, the regulatory mechanism underlying lipid metabolism during strawberry development and ripening remains unclear. Although LEAFY COTYLEDON 2 (LEC2) regulates lipid metabolism, its function in strawberries is unknown. This study explored the role of FaLEC2 in strawberries. FaLEC2 is highly expressed during strawberry development. FaLEC2 overexpression delayed ripening, promoted the accumulation of phosphatidylcholines (PCs), including 1,2-dipalmitoyl-3-sn-phosphatidylcholine (DPPC) and PC (16:0/18:3(9Z,12Z,15Z)), and linoleic acid (LA), upregulated FaLOX2.4/2.5/3, downregulated FaLOX5-like2, and regulated various transcription factors (TFs). Correlation analysis revealed associations among lipid metabolites and multiple regulatory genes. Yeast one-hybrid and dual-luciferase reporter assays preliminarily suggested a regulatory association between FaLEC2 and FaPLA1, identifying FaPLA1 as a putative direct target. These findings indicate that FaLEC2 modulates PCs and LA by regulating lipid-related genes, along with a set of candidate TFs, thereby contributing to the regulation of phospholipid and linoleic acid metabolism in strawberries.
Hongyan Lu, Qiling Yu, Mengyan Li et al.· Journal of Agricultural and...· 0 citations
The combined transcriptome and metabolome analysis revealed that plant hormone signal transduction, phenylpropanoid biosynthesis, and flavonoid biosynthesis were significantly enriched in resistant rice varieties, providing valuable information on the molecular mechanisms by which rice defends against U. virens infection.
Rongtao Fu, Huan Li, Xi Luo et al.· BMC Plant Biology· 0 citations
This study provides a validated CRISPRi workflow for inducible repression of msmeg_6073 in M. smegmatis, suggesting that msmeg_6073 repression may be condition dependent, and improves understanding of the mycobacterial epitranscriptome.
Ali Aadel Karimpour, Suwatchareeporn Rotcheewaphan, Pornchai Kaewsapsak· Journal of Medical Bioscienc...· 0 citations
Results provide a genetic entry point for metabolic engineering and breeding efforts aimed at modulating grain phenolic profiles and indicate that SbmiR397-5p negatively regulates the expression of SbLAC14 in relation to CT biosynthesis, identifying it as a potential target for manipulating CT metabolism in sorghum.
Yannan Shi, Yongchao Guo, Jinping Wang et al.· Plants· 0 citations
It is demonstrated that the Smi-miR164a module acts as a pivotal regulator, positively influencing phenolic acid biosynthesis while negatively regulating tanshinone production in S. miltiorrhiza.
Huaqian You, Weibo Jin, Dongfeng Yang et al.· Frontiers in Plant Science· 0 citations