Identification of reproductive and molecular biomarkers associated with clinical metritis in dromedary camels (Camelus dromedarius)
TL;DR
Clinical metritis in dromedary camels was associated with significant hematological, metabolic, hormonal, oxidative, immunological, and genetic alterations, and these findings should be interpreted with caution and require validation in larger, independent camel populations.
Abstract
Background Metritis is a major cause of infertility, contributing to repeat breeding, early embryonic loss, fetal mortality, and abortion. Methods This study investigated alterations in metabolic, hormonal, oxidative, immunological, and molecular markers associated with metritis susceptibility in dromedary camels (Camelus dromedarius). A total of 100 non-lactating multiparous females (87 healthy and 13 metritic) were examined. Blood samples were collected and analyzed to evaluate hematobiochemical parameters, inflammatory and antioxidant markers, and single nucleotide polymorphisms (SNPs) in selected immune- and antioxidant-related genes. Results Metritic animals exhibited fever and abnormal uterine discharge. Gene expression analysis showed significant upregulation of CARD9, SIGLEC1, VSIG4, BTRC, SLC2A3, ACOD1, and PFKFB3, alongside downregulation of CPT1A, ATF4, GADD45B, SLC7A11, and PRDX3. Sequencing identified 19 SNPs, with significant differences in genotype distribution between groups, and discriminant analysis achieved complete classification accuracy. Hematological findings included normocytic normochromic anemia, leukocytosis, neutrophilia, lymphopenia, and monocytosis. Biochemically, metritic camels showed hypoglycemia and decreased levels of estrogen, progesterone, FSH, LH, T4, calcium, and antioxidant markers (GSH, CAT, and TAC), while NEFA, BHBA, cortisol, MDA, and pro-inflammatory cytokines (IL1α, IL1β, IL6, IL10, and TNFα) were significantly elevated. Conclusion Overall, clinical metritis in dromedary camels was associated with significant hematological, metabolic, hormonal, oxidative, immunological, and genetic alterations. These findings identify a panel of reproductive and molecular biomarkers that may serve as promising candidate biomarkers associated with clinical metritis. However, given the observational nature of the study and the relatively small number of metritic she-camels, these findings should be interpreted with caution and require validation in larger, independent camel populations before their diagnostic, prognostic, or breeding applications can be established.