Open access

Tailoring Precise Genomic Integration Toward Isolate-to-Industry Strain Development for Scalable High-Titer Production of Polyhydroxyalkanoate

Peng Liu Xinying Xie Yi-Hao Deng Ze-Feng Li Chi Wang Hao Yang Yu-xi Li Ling-Ling Zhao W. Situ Hong-Wei Shen Liu-Song Yu Jin-Yan Lv Yan Xiao Yi-Na Lin Jian-Wen Ye

Jul 2026 · bioRxiv · 0 citations · 99 references

Biology

TL;DR

This study demonstrates a successful paradigm for engineering a newly isolated strain toward robust, high-titer and cost-competitive PHA production across lab-to-industry scales.

DOI Semantic Scholar

Read PDF

Similar papers

#gene editing Sep 2026

Highly efficient production of 1,3-propanediol by CRISPR-Cas12a-edited Klebsiella pneumoniae using glycerol and lignocellulosic hydrolysate as co-substrates.

Efficient biosynthesis of 1,3-PDO, a key bio-based chemical, depends on precise regulation of the host metabolic network. In this study, a heterologous CRISPR-Cas12a genome editing system was established and systematically optimized in Klebsiella pneumoniae, enabling efficient and stable genome editing (75-100% efficie...

Li Wang, Ming-Yang Zhao, Yuan-Ming Ye et al. · Bioresource Technology · 0 citations

Aug 2026

Reprogramming Komagataella phaffii Cell Factory for High-Yield Production of 3S,3′S-Astaxanthin via Multiplex Metabolic Engineering and Its Application

The synthesis of 3S,3′S-astaxanthin was successfully and effectively applied in shrimp farming for color enhancement and antioxidant effects and will pave the way for astaxanthin industrial production.

Xinying Zhang, Ji-Yuan Hu, Yan-Xuan Wu et al. · ACS Synthetic Biology · 0 citations

Sep 2026

High-Level Terpene Production via a Novel Actinomycetota-Derived MVA Pathway in E. coli.

A genomically integrated Mevalonate (MVA) pathway from Actinomycetota in E. coli BL21(DE3) is developed to enhance terpene precursor supply and provides potential reservoir of diverse chassis for the industrial production of terpenoid-derived compounds.

Wenchao Liu, Xueying Tian, Wai-Chuen Wong et al. · Metabolic Engineering · 0 citations

Aug 2026

A Dual-Dimensional CRISPR Toolkit Enables One-Step High-Efficiency Multiplex Genome Editing in Komagataella phaffii.

A CRISPR toolkit - Efficient Multi-Gene Editing System 3.0 (EMGES 3.0) - which enabled one-step large-fragment integration coupled with multiplex gene knockout coupled with multiplex gene knockout is established and provides a robust technical foundation for complex compounds biosynthesis and high-yield industrial stra...

Zhixian Yu, Kaidi Chen, Gulikezi Maimaitirexiati et al. · Metabolic Engineering · 0 citations

Open access Jul 2026

Systematic engineering of Pichia pastoris for high-level production of recombinant human granulocyte-macrophage colony-stimulating factor.

This work demonstrates that the high-yield production of recombinant human therapeutic glycoproteins bearing homogeneous, humanized N-glycans in engineered yeast offers promising prospects.

Zhenzhen Cheng, Keyu Gong, Yuchao Song et al. · Bioresource Technology · 0 citations

Review Sep 2026

Multidimensional metabolic engineering strategies for efficient production of branched-chain amino acids and their derivatives in Escherichia coli and Corynebacterium glutamicum.

This review presents a comprehensive analysis of recent advances in microbial BCAAs production through a Mechanism-Module-Process framework, providing a holistic roadmap for constructing superior BCAAs cell factories and extending their metabolic potential toward derivative biosynthesis.

Guihong Zhao, Si-Yu Tian, Jiong-Ran Li et al. · Biotechnology Advances · 0 citations