Skip to content
Log in
Sponsored AI AM CLAUDE, A Beginner's Guide: Learn the basics of working with AI AI AM CLAUDE, A Beginner's Guide: Learn the basics of working with AI

Rapid and efficient generation of human 8-cell-like cells for embryo modelling

Arda Odabas Sıla Unlu Eren Ozturk Nilya Karasurmeli Kelly Hu Marion Leleu Can Aztekin Tamer T. Onder
Aug 2026 · bioRxiv · 0 citations
Biology

TL;DR

Ri8CLC induction enables blastoid formation even in the absence of MEK inhibition, TGF-β/Activin/Activin/Nodal inhibition and exogenous LIF, revealing a developmental competence consistent with an early embryonic state and providing a tractable model for studying early human embryogenesis.

DOI Semantic Scholar
View source

Similar papers

Review Sep 2026

Generation of eight-cell embryo-like cells from human pluripotent stem cells.

The approach provides a valuable model for studying human early embryogenesis, particularly the molecular events surrounding ZGA, and is expected that an individual with ~1 year of experience working with human PSC culture would be able to carry out this protocol.

Md. Abdul Mazid, Yun-Pan Li, Min-Die Zhao et al. · 0 citations
Open access Aug 2026

Pcgf5 controls the exit from totipotency in mouse embryonic stem cells.

It is shown that among the six Pcgf paralogs, Pcgf5, which is most prominently upregulated in 2CLCs and forms an MERVL-driven chimeric transcript (Pcgf5MT2C_Mm) during ZGA in 2-cell embryos, controls exit from the 2C-like state in mouse ESCs.

Satoshi Mashiko, Shinnosuke Honda, Shunta Ikeda et al. · 0 citations
Sep 2026

Msl3-mediated H4K16ac suppresses 2C-like reprogramming by sustaining LINE1 expression in mouse embryonic stem cells.

Two-cell-like cells (2CLCs), a rare totipotent-like population within mouse embryonic stem cells (mESCs) cultures, serve as a valuable model for studying early embryonic events like zygotic genome activation. However, the molecular mechanisms restraining their spontaneous emergence remain elusive. Here, we identify Msl...

Yu-Qing Zhu, Shuo Cao, Qing-Ying Wang et al. · 0 citations
Open access Sep 2026

Long-Term Labeling and Differentiation Monitoring of Mouse Spermatogonial Stem Cells Using CellREADR

This work identified several previously unreported features of CellREADR, including RNA interference induced by double-strand RNA formation that is obligatorily generated during the normal operation of the CellREADR system, and established its application for long-term labeling of mSSCs and monitoring mSSC differentiat...

Shuai-Tao Hu, Lin Yan, Gulistan Khan et al. · 0 citations
Open access Sep 2026

High-throughput functional characterization of enhancers in totipotent-like cells

Zygotic genome activation (ZGA) marks the initial transcription event in embryogenesis, yet the cis-regulatory mechanism remains unclear. Here, utilizing massively parallel reporter assays, we functionally dissect enhancers across mouse genome in DUX-induced 2C-like cells (2CLCs). Through integrated analysis with epige...

Ling-Yu Yang, Tian-Ran Peng, Ya-Ting Zhu et al. · 0 citations
Open access Sep 2026

Multiplexed embryo profiling links cellular state to zygotic genome activation in single cells

The cell cycle phase is the major source of variability in transcription within a division cycle, and combining this with the analysis of key transcription factors and chromatin modifier state enables accurate prediction of transcriptional output during zygotic genome activation in individual cells.

Max Hess, M. Wyss, Edlyn Wu et al. · 0 citations

We use cookies to run the site and, with your consent, for analytics and to show ads. See our Cookie Policy.