Development of a novel phage-displayed fusion protein of newcastle disease virus as a highly specific antigen for serological assays.
TL;DR
The displayed antigen successfully detected NDV-GVII-specific antibodies in vaccinated chickens and addressed safety concerns associated with wild-type virulent NDVs and offers improved diagnostic accuracy and scalability, aligning with industry needs for improved Newcastle disease detection, control, and management on poultry farms.
Abstract
Newcastle disease virus is one of the most highly contagious infections impacting poultry industries worldwide. This study aimed to develop a bacteriophage display system as a cost-effective and immunogenic method for expressing the immunodominant region of the Newcastle Disease Virus Fusion (F) protein for use in serological assays. Both in-silico and in-vitro analyses indicated that the selected antigen is stable, accurate, and reliable for further in-vivo studies. The displayed antigen successfully detected NDV-GVII-specific antibodies in vaccinated chickens. Additionally, a significant correlation was observed between ELISA and HI test results on chicken serum samples. This approach addresses safety concerns associated with wild-type virulent NDVs and offers improved diagnostic accuracy and scalability, aligning with industry needs for improved Newcastle disease detection, control, and management on poultry farms.